The Characteristics and qRT-PCR Analysis of Ten Candidate Effector Proteins Induced by Puccinia triticina
ZHANG Yue1,*, LI Jian-Yuan1,2,*, ZHANG Na1, WEI Xue-Jun1, YANG Wen-Xiang1,**, LIU Da-Qun1,3,**
1 College of Plant Protection, Hebei Agricultural University/ Technological Innovation Center for Biological Control of Crop Diseases and Insect Pests of Hebei Province, Baoding 071001, China; 2 Xingtai College, Xingtai 054001, China; 3 Graduate school of Chinese Academy of Agricultural Sciences, Beijing 100081, China
Abstract：Wheat leaf rust, caused by Puccinia triticina (Pt), is one of the most destructive diseases of wheat. The haustoria of Pt is the main place where the effector proteins were secreted, and the effector protein is the key of the pathogenetic of Pt. To clarify the expression characteristics of these effector proteins in the stage of interaction with the host, 635 candidate effector proteins have been harvested with bioinformatics analysis based on the sequencing of transcriptome. qRT-PCR analysis was used to test the expression characters of 10 candidate effector proteins. The results showed that these genes were consistent with the basic structural properties of fungi effector proteins and were highly homologous to wheat leaf rust BBBD and wheat stem rust (P. graminis f.sp. tritici), three of these genes contained a known conserved domain [Y/F/W]xC, one gene contained [L/I]xAR domain, two genes were glycosyl hydrolase family, and one gene had dioxygenase activity. Six of them was induced to be up-regulated in the late stage and the others were up-regulated dring 6 hpi to 48 hpi respectively. The results lay a foundation for revealing the interaction mechanism between wheat and leaf rust, and also provides favorable conditions for the study of wheat leaf rust effector proteins.
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