Screening and Identification of 3 Actinobacterial Strains Against Tobacco Bacterial Wilt
WU Xiang*, TAN Hao, PENG Wei-Hong
Soil and Fertilizer Institute, Sichuan Academy of Agricultural Sciences/Scientific Observing and Experimental Station of Southwestern Region Agromicrobiological Resource Utilization, Ministry of Agriculture/Key Laboratory of Agricultural Environment in Southwest Mountain Areas, Ministry of Agriculture, Chengdu 610066, China
Abstract：At present, bacteria are the main microorganisms to inhibit tobacco bacterial wilt, and Actinobacteria are few. In this study, Actinobacteria, which can be used to develop and produce bio organic fertilizer for controlling tobacco bacterial wilt, were screened to determine their taxonomic status. Firstly, antagonistic Actinobacteria against tobacco bacterial wilt were screened from tobacco (Nicotiana tabacum) rhizosphere soil by the method of inhibition zone assessment. The taxonomic status of antagonistic Actinobacteria was determined by polyphase analysis of phenotypic and physio biochemical characterizations, in combination with genetic characteristics. The results showed that 3 Actinobacterial strains with good antagonistic effect against tobacco bacterial wilt were obtained, and the 3 Actinobacterial strains could inhibit the pathogen of tobacco brown spot disease (Alternaria alternata) and other bacterial wilt pathogens of Solanaceae plants such as capsicum (Capsicum annuum), tomato (Solanum lycopersicum) and potato (Solanum tuberosum). In the 3 strains, SC-105-B-10 (GenBank No. KT633863) belonged to Streptomyces microflavus, L2-003-A-5 (GenBank No. KT119348) belonged to Streptomyces albidoflavus, and SC-168-A-2 belonged to Streptomyces pratensis (GenBank No. KR140205). This study obtained 3 Actinobacterial strains, which have potential for the development of bio-organic fertilizer for controlling tobacco bacterial wilt.
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