Identification and Biological Characteristics of Pathogens in Potato Common Scab
LI Chi1, LIU Yan1, LIANG Yan1, XING Xing1, ZHANG Jian-Ping3, LI De-Zhou1, 2, *, ZHANG Xiao-Yu1, *
1 College of Agronomy, Inner Mongolia Agricultural University, Hohhot 010019, China;
2 Development Planning Office, Inner Mongolia Agricultural University, Hohhot 010018, China;
3 Institute of Plant Protection, Inner Mongolia Academy of Agriculture and Animal Husbandry, Hohhot 010031, China
摘要马铃薯疮痂病(potato common scab)在内蒙古西部种植区发生严重,是马铃薯(Solanum tuberosum)生产的主要障碍。为明确内蒙古武川县马铃薯疮痂病病原菌种类和生物学特性,本研究采用稀释分离法对病原菌进行了分离,得到1株分离物PS1。以马铃薯片法和盆栽接种进行致病性测定,并利用特异性引物检测了分离物的致病基因,结果表明,该菌株能在马铃薯片上定殖并使薯片产生褐色坏死,使新生块茎产生典型的疮痂病症状,并检测到该菌株中含有毒素合成基因(thaxtomin A and B, txtAB)、致病性因子基因(tomatinase, tomA)和诱导坏死蛋白基因(necrosis-inducing protein, nec1)3个毒素致病基因。通过形态学特征、生理生化特性及16S rDNA序列分析进行鉴定,该菌株为加利利链霉菌(Streptomyces galilaeus)。生物学特性研究结果显示,该菌株的生长最适培养基为燕麦液体培养基,培养10 d时,菌丝干重达到最大,最适pH为7,最适培养温度为32 ℃,最适单一碳源为葡萄糖,最适单一氮源为L-甲硫氨酸。本研究明确了武川地区马铃薯疮痂病病菌菌株PS1的种类和生物学特性,为进一步研究其流行规律及防治措施提供依据。
Abstract：Potato common scab by Streptomyces spp.is a serious disease occurring within the western growing region of Inner Mongolia and is a major obstacle to potato (Solanum tuberosum) production.In order to clarify the pathogens and biological characteristics of potato common scab in Wuchuan county, Inner Mongolia, the pathogens were isolated by dilution separation method, and one isolate PS1 was obtained.The pathogenicity was determined by potato chip method and potted inoculation, and the pathogenic genes were detected by specific primers.In results, The strain PS1 could colonize in potato chips and produce brown necrosis, and produce typical scab symptoms in potted potato tubers.The pathogenic genes txtAB, tomA and nec1 (necrosis-inducing protein) were detected.The morphological characteristics, physiological and biochemical characteristics and 16S rDNA sequence were analyzed to identify the pathogens, and the isolate was identified as Streptomyces galilaeus.The results of biological characteristics indicated that the oat liquid medium for mycelium growth was optimum.When cultivated 10 d the mycelium dry weight was maximum.The optimum pH was 7, and the optimum cultivation temperature was 32 ℃.The optimum single carbon and nitrogen source were glucose and L-methionine.In this study, the pathogen species and biological characteristics in potato common scab were clarified, which laid the foundation for further study of epidemic patterns an control measures.
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