Abstract：China is one of the main consumer of genetically modified soybean (Glycine max) and the safety of genetically modified soybeans received wider attention. It is imperative to study fast and accurate detection methods for genetically modified ingredients in soybeans. In this study, based on TaqMan probe technology, a duplex real-time PCR method was established to detect MON87701 and MON87708 transgenic soybean strains simultaneously, and its specificity, sensitivity and applicability were analyzed. The results showed that the method could accurately detect target genes from 18 transgenic samples and 5 non-transgenic samples, indicating good specificity. The sensitivity test showed that the sensitivity of this method was 0.01%. The applicability of this method had been tested by 10 real samples and the results were as same as the EU standard. These results suggested that the duplex real-time PCR method was an effective tool for detection of soybean MON87701 and soybean MON8770 in the exit and entry inspection laboratory. This study provides a reference for the regulation of genetically modified products.
袁俊杰, 魏霜, 龙阳, 吴希阳, 李想, 付伟. 转基因大豆MON87701和MON87708双重实时荧光PCR检测技术的建立与应用[J]. 农业生物技术学报, 2020, 28(2): 342-348.
YUAN Jun-Jie, WEI Shuang, LONG Yang, WU Xi-Yang, LI Xiang, FU Wei. Development and Application of Duplex Real-time PCR Assay for the Detection of Genetically Modified Soybean (Glycine max) MON87701 and MON87708. 农业生物技术学报, 2020, 28(2): 342-348.
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