Ultrastructure of Marc-145 Cells in Autophagy Induced by Porcine reproductive and respiratory syndrome virus
LI Xiao-Jing1, GONG Shuang-Yan1, LI You-You1, CHEN Ying-Qi1, CAI Yao1, LI Yu-Meng1, XU Yi-Fei1, XU Zhi-Wen1,2,*, ZHU Ling1,2
1 College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China; 2 Key Laboratory of Animal Diseases and Human Health of Sichuan Province, Chengdu 611130, China
Abstract：Autophagy is one of the basic researches of viral infection and tumor development. In order to understand the characteristics of autophagosomes, the determination of autophagosomes and the ultrastructure of cells, this experiment used Porcine reproductive and respiratory syndrome virus (PRRSV) to induce autophagy in African green monkey (Cercopithecus aethiops) embryonic kidney cells Marc-145. The cells and autophagosomes were observed by transmission electron microscopy and fluorescence microscope. The occurrence of autophagy was initially determined, and changes in structures such as lysosomes, endoplasmic reticulum, mitochondria and nucleus were observed. Marc-145 cells changed from a typical fusiform to a round shape after PRRSV infection, and there were significant autophagosomes in the infected cells. Ultrastructural changes such as mitochondrial ridge dissolution, endoplasmic reticulum degranulation, lysosomal activity enhancement, and nuclear abnormalities were observed during ultrastructural observation. The results showed that PRRSV induced autophagy in Marc-145 cells and the degree of autophagy were closely related to ultrastructure. The ultrastructural state could be used as an important reference for determining the development of autophagy. The results showed that PRRSV induced autophagy in Marc-145 cells, the degree of autophagy was closely related to the ultrastructure, and the state of the ultrastructure could be used as an important reference for judging the development of autophagy. This study could provide important reference for the study of autophagy, cell ultrastructure and viral infection.
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