Genetic Diversity Analysis of Major Indigenous Chicken (Gallus gallus) Breeds in Zhejiang Province
WANG Zhen-Zhen1, 2, *, HUANG Ling-Ling2, 3, *, TIAN Yong2, YU Zao-Sheng4, SHI Meng-Da5, ZENG Tao2, LU Li-Zhi1, 2, **
1 College of Chemistry and Life Science, Zhejiang Normal University, Jinhua 321004, China; 2 Institute of Animal Husbandry and Veterinary Science, Zhejiang Academy of Agricultural Science, Hangzhou 310021, China; 3 College of Animal Science and Technology, Fujian Agriculture and Forestry University, Fujian 350002, China; 4 Songyang County Zao Shang Hao Queshan Chicken Breeding Co., Ltd., Lishui 323000, China; 5 Xinchang County Gongting Huang Chicken Breeding Co., Ltd., Shaoxing 312530, China
Abstract：Indigenous chicken (Gallus gallus) breeds are important part of China's poultry genetic resources. Zhejiang province has a long history of breeding chicken and has various chicken genetic resources. In order to study the genetic diversity and genetic structure of main chicken breeds in Zhejiang province and promote the protection and rational development and utilization of different chicken breeds resource, the present study used short tandem repeat (STR) typing technique to detect the genetic diversity of 6 chicken breeds Queshan chicken, Xianju chicken, Xiaoshan chicken, Silky chicken, Longyou Ma chicken, and Baier Huang chicken (total of 358) at 26 microsatellite loci. The results showed that 153 effective alleles were detected at 26 sites in 6 populations, and the average values of effective number of alleles (Ne), expected heterozygousity (He), gene flow (Nm) and Shannon information index I were 5.906 9, 0.795 9, 2.007 and 1.925 1, respectively, and the polymorphism information content (PIC) was 0.24 to 0.87. Nei genetic distance was 0.103 5~0.380 9, among which the genetic distance between Queshan chicken and Xiaoshan chicken was the farthest (0.3809), followed by Queshan chicken and Baier Huang chicken (0.1072), and the genetic distance of Longyou Ma chicken and Xianju chicken was the smallest (0.1035). The results of cluster analysis showed that Queshan chicken and Baier Huang chicken were a single cluster and Xianju chicken and Longyou Ma chicken were a group. All the 6 groups had high genetic diversity, among which the Baier Huang chicken had the highest genetic diversity, the Silky chicken had the lowest genetic diversity, and the similarity order of Queshan chicken with other chicken breeds was Baier Huang chicken, Silky chicken, Longyou Ma chicken, Xianju chicken and Xiaoshan chicken consecutively. In this study, the genetic diversity of 6 chicken breeds in Zhejiang province was analyzed, and the genetic structure of the main chicken breeds in Zhejiang province was further improved, which might provide basic data for the development and utilization of chicken genetic resources.
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