Effects of FecB Mutation on Follicular Development and Expression of AMH and AMHR2 Genes in Small Tail Han Sheep (Ovis aries)
TAO Lin1, *, GUO Xiao-Fei2, *, WEN Yu-Liang1, DI Ran1, LIU Qiu-Yue1, HU Wen-Ping1, ZHANG Xiao-Sheng2, ZHANG Jin-Long2, WANG Xiang-Yu1, **, CHU Ming-Xing1, **
1 Key Laboratory of Animal Genetics, Breeding and Reproduction of Ministry of Agriculture, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China; 2 Tianjin Institute of Animal Sciences, Tianjin 300381, China
Abstract：As an excellent domestic breed with fecundity, Small Tail Han sheep (Ovis aries) could get high ovulation rate and little size as a result of A476G mutation in bone morphogenetic protein receptor 1B (BMPR1B) gene (FecB mutation), under which the mechanism remains to poorly understand. The objective of this study was to investigate the effects on the number and diameter of follicle and the expression patterns of anti-Müllerian hormone (AMH) gene and its receptor Ⅱ (anti-Müllerian hormone receptor Ⅱ, AMHR2) gene in Small Tail Han sheep with or without FecB mutation (mutant homozygous BB and wide type ++, respectively). Based on TaqMan probe genotyping and estrus synchronization, the number and diameter of follicle over 3 mm from ++ (n=21) and BB ewes (n=16) were recorded. qRT-PCR technology was applied to detect the expression of AMH and AMHR2 genes in 14 tissues (hypothalamus, pituitary, ovary, heart, liver, spleen, lung, kidney, brain, adrenal gland, small intestine, oviduct, uterus, thyroid) from 3 BB ewes during follicular phase and 3 tissues (hypothalamus, pituitary and ovary) from BB and ++ ewes during luteal phase and follicular phase (n=3, 3, 3, 3, respectively). The results revealed that FecB mutation had a highly significant increase in number of follicle (P<0.01) and a highly remarkable decrease in follicular diameter (P<0.01). Two genes were expressed in 14 tissues of BB ewes during follicular phase, wherein both genes expressed highly in hypothalamus-pituitary-ovary gonad axis as well as AMH gene expressed highest in ovary and AMHR2 gene in adrenal gland. The expression of AMH gene in hypothalamus-pituitary-ovary gonad axis between BB and ++ animals during different phases of follicular development were not significantly different (P>0.05). There was no significant difference for the expression of AMHR2 gene in ovary between 2 categories of animals during different phases of follicular development (P>0.05). The expression of AMHR2 gene in pituitary of BB ewes was higher than ++ contemporaries (P<0.01). Stage of follicular development and FecB genotype had a highly significant effect on expression of AMHR2 gene in hypothalamus (P<0.01). A conclusion can be drew that FecB mutation caused the change of follicular development and expression of AMHR2 gene in time and space in Small Tail Han sheep and the special expression of AMHR2 gene in hypothalamus-pituitary-ovary gonad axis system may be involved in the regulation that FecB mutation alters follicular development, which would give novel theoretical basis to multiparous meat sheep breeding in our country.
陶林, 郭晓飞, 文禹粱, 狄冉, 刘秋月, 胡文萍, 张效生, 张金龙, 王翔宇, 储明星. FecB突变对小尾寒羊卵泡发育及AMH和AMHR2基因表达的影响[J]. 农业生物技术学报, 2019, 27(4): 684-691.
TAO Lin, GUO Xiao-Fei, WEN Yu-Liang, DI Ran, LIU Qiu-Yue, HU Wen-Ping, ZHANG Xiao-Sheng, ZHANG Jin-Long, WANG Xiang-Yu, CHU Ming-Xing. Effects of FecB Mutation on Follicular Development and Expression of AMH and AMHR2 Genes in Small Tail Han Sheep (Ovis aries). 农业生物技术学报, 2019, 27(4): 684-691.
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