Abstract：Bacitracin methylene disalicylate (BMD), as the second generation of bacitracin feed additive, can promote the growth and improve the immunity of livestock. This study aims to investigate the dynamic effect of BMD on the immune function of rabbits, 300 New Zealand weanling rabbits (35-day-old) were divided into BMD high (BMD H, 100 mg/kg), BMD middle (BMD M, 50 mg/kg), BMD low dosage group (BMD L, 25 mg/kg), Bacitracin Zinc group (BZ, 50 mg/kg), and blank control group (C, no addition). Studies in this group have confirmed that BMD could promote the growth of rabbits. At the end of the 35-day trial, the contents and mRNA expression of interleukin 2 (IL-2) and immunoglobulin G (IgG) in cecum and duodenum were detected by enzyme linked immunosorbent assay (ELISA) and qRT-PCR assay. The contents of IgG and IL-2 in cecum in BMD and BZ group were significantly higher than C group (P＜0.05). The contents of IL-2 in duodenum in BMD H group were significantly higher than that C group (P＜0.05). The correlation analysis showed that the contents of IL-2 and IgG in the cecum were positively correlated with the gene expression levels (P＜0.05). The results showed that the addition of BMD to diets could increase the content of IL-2 and IgG, and upregulate mRNA expression. The effect of 50~100 mg/kg BMD in dietary supplementation is equivalent to the effect of BZ. This study further confirmed that BMD could enhance the immunity of rabbits, and provide evidence for its application in rabbit industry.
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