Abstract：Glucocorticoid receptor (GR) is a member of the conservative nuclear receptor superfamily. It belongs to nuclear transcription factor. It plays an important role in maintaining the stability of the body and animal breeding and metabolism. The aim of this experiment was to clone the cDNA full length of GR gene of pigeon (Columba livia domestica) and analyze its expression profile. The specific primers were designed based on the published necleotide sequence of chicken (Gallus gallus domesticus) . The sequencing results showed that GR gene containing 2 313 bp CDS, 102 bp 5'UTR and 18 bp 3'UTR, encoding a protein of 770 amino acids(GenBank accession No: NM_001037826.1). The sequence homology analysis showed that the pigeon GR gene shared 94%, 85% and 75% homology of the amino acid sequence with chicken, crocodlie (Alligator mississippienses) and human (Homo sapiens), respectively. The amino acid sequence analysis of Chicken, crocodile and human found the GR amino acid sequence had a very conserved DNA binding domain (DBD), and a higher conserved hormone recognition domain (LBD), in addition to a GR specific region. qRT-PCR results suggested that GR mRNA were expressed in the different organizations. And the expression quantity from high to low were testis, pancreas, lung, ovary, abdominal fat, liver, heart, skeletal muscle, kidney, spleen, oviduct, stomach, hypothalamus, brain, intestine and craw successively. The expression of GR gene in the testis was significantly higher than other tissues (P＜0.05), followed by the pancreas and lung (P＜0.05). In this study, we obtained the full length of GR gene cDNA and the expression of GR gene, this results provide a basic data for further study on the function of GR gene.
[1]张晓燕, 赵海谕, 张思欢, 等.2015. 奶山羊垂体同型框转录因子2基因(PITX2)分析克隆、序列分析及其mRNA表达规律[J]. 农业生物技术学报, 23(7):905-916. (Zhang X Y, Zhao H Y, Zhang S H, Zhao H Y, Zhang S H, et al. 2015. Molecular cloning, sequence analysis and mRNA expression profiling of paired-liked homodomain transcription factor 2 gene(PITX2) in dairy goat(Capra hircus)[J]. Journal of Agricultural Biotechnology, 23(7):905-916.)[2]Black B E, Holaska J M, Rastinejad F, et al.2001. DNA binding domains in diverse nuclear receptors function as nuclear export signals[J]. Current Biology, 11(22): 1749-1758.[3]Challis J R, Sloboda D, Matthews S G, et al.2001. The fetal placental hypothalamic-pituitary-adrenal (HPA) axis, parturition and post natal health[J]. Molecular Cell Endocrinology, 185(1-2): 135-144.[4]Darmaun D, Matthews D E, Bier D M.1988. Physiological hypercortisolemia increases proteolysis, glutamine, and alanine production[J]. American Journal of Physiology, 255(3): E366-373.[5]Feldman S, Weidenfeld J.2001. Electrical stimulation of the dorsal hippocampus caused a long lasting inhibition of ACTH and adrenocortical responses to photic stimuli in freely moving rats[J]. Brain Research, 911(1): 22-26.[6]Jia X C, Ny T, Hsueh A J.1990. Synergistic effect of glucocorticoids and androgens on the hormonal induction of tissue plasminogen activator activity and messenger ribonucleic acid levels in granulosa cells[J]. Molecular and Cellular Endocrinology, 68(2-3): 143-151.[7]Kwok A H, Wang Y, Wang C Y, et al.2007. Cloning of chicken glucocorticoid receptor (GR) and characterization of its expression in pituitary and extrapituitary tissues[J]. Poultry Science, 86(2): 423-430.[8]Lamounier Z V, Ehrhart BM.2006. Fat tissue metabolism and adrenal steroid secretion[J]. Current Hypertension Reports, 8(1): 30-34.[9]Lan X Y, Penagaricano F, Dejung L, et al.2013a. A missense mutation in the PROP1 (prophet of pit 1) gene affects male ferility and milk production traits in the US Holstein population[J]. Journal of Dairy Science, 96(2):1255-1257.[10]Marcel J M, Schaaf M, Cidlowski JA .2003. Molecular mechanisms of glucocorticoid action and resistance [J]. Journal of Steroid Biochemistry and Molecular Biology, 83(1-5): 37-48.[11]Michael A E, Gregory L, Walker S M, et al.1993. Ovarian 11 beta-hydroxysteroid dehydrogenase: potential predictor of conception by in-vitro fertilisation and embryo transfer[J]. Lancet, 342(8873): 711-712.[12]Michael A E, Pester L A, Curtis P, et al.1993. Direct inhibition of ovarian steroidogenesis by cortisol and the modulatory role of 11 beta-hydroxysteroid dehydrogenase[J]. Clinical Endocrinology, 38(6): 641-644.[13]Proszkowiec W M, Porter T E.2010. Functional characterization of chicken glucocorticoid and mineralocorticoid receptors[J]. American Journal of Physiology Regulatory Integrative and Comparative Physiology, 298(5): R1257-1268.[14]Pujols L, Mullol J, Torrego A, et al.2004. Glucocorticoid receptors in human airways[J]. Allergy, 59(10):1042-1052[15]Rouleau G, Karpati G, Carpenter S, et al.1987. Glucocorticoid excess induces preferential depletion of myosin in denervated skeletal muscle fibers[J]. Muscle Nerve, 10(5): 428-438.[16]Sanders M M, McKnight G S.1985. Chicken egg white genes: multihormonal regulation in a primary cell culture system[J]. Endocrinology, 116(1): 398-405.[17]Stewart P M, Mason J I.1995. Cortisol to cortisone: glucocorticoid to mineralocorticoid[J]. Steroids, 60(1): 143-146.
