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2025年8月1日 星期五
  2015, Vol. 23 Issue (1): 96-106    
  研究论文与报告 本期目录 | 过刊浏览 | 高级检索 |
金针菇种质资源5个农艺性状与SSR标记的关联分析
陆欢1,张丹1,章炉军1,王瑞娟1,尚晓冬1,谭琦2
1. 上海市农业科学院
2. 上海市农业科学院食用菌研究所
Association Analysis of Five Agronomic Traits with SSR Markers in Flammulina velutipes Germplasm
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摘要 金针菇(Flammulina velutipes)是一种重要的商业化栽培食用菌,为挖掘控制金针菇重要农艺性状的基因组区段,本研究以90份金针菇种质资源为实验材料,测定其原基期、菌柄直径、菌柄长度、菌盖直径和单瓶产量5个农艺性状,利用全基因组序列开发的95份多态简单重复序列(simple sequence repeat, SSR)标记对供试材料进行基因组扫描,在分析实验材料的群体结构和连锁不平衡基础上,采用一般线性模型方法对5个农艺性状进行关联分析。结果表明,各供试材料的5个农艺性状均有极显著差异。95份多态SSR标记共检测到582个等位变异,平均每个位点的等位变异数为6.13个。标记多态信息含量(polymorphism information content, PIC)变化范围为0.204~0.818,平均为0.542。群体遗传结构分析将供试材料分为8个亚群体,群体内SSR位点间存在较高的连锁不平衡(linkage disequilibrium, LD),不平衡程度D'值大于0.5的组合数占总组合数的33.01%。关联分析检测出28个标记与5个性状显著相关,其中原基期的关联标记有2个,菌柄直径的关联标记有12个,菌柄长度的关联标记有3个,菌盖直径的关联标记有9个,单瓶产量的关联标记有5个。关联SSR标记对表型变异解释率的变幅为5.11%~23.55%。 研究表明,所选金针菇种质资源群体的遗传多样性以及连锁不平衡程度较高,适用于关联分析研究。本研究结果对金针菇核心种质资源库构建以及分子标记辅助育种具有参考价值。
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陆欢
张丹
章炉军
王瑞娟
尚晓冬
谭琦
关键词 金针菇简单重复序列(SSR)群体结构连锁不平衡(LD)关联分析    
Abstract:Flammulina velutipes is an important commercial edible fungus. Exploring the genomic regions controlling important agronomic traits is helpful to the genetic improvement of F. velutipes. A total of 90 F. velutipes strains were used as tested materials, and 5 agronomic traits including primordium period, stipe diameter, stipe length, pileus diameter and yield per bottle were detected. Total 95 polymorphism simple sequence repeat(SSR) markers that developed from the whole genome sequence of F. velutipes were used to scan the genome of materials in present study. On the basis of analyzing population structure and linkage disequilibrium (LD) of the experimental materials. The association analysis between 5 agronomic traits and SSR loci were performed by using GLM (general linear model) program in TASSEL software. The results showed that the differences between the corresponding agronomic traits of the materials were highly significant(P<0.01). A total of 582 alleles were found among 95 pairs of SSR markers with 6.13 alleles per locus. The polymorphism information content (PIC) value ranged from 0.204 to 0.818, with an average of 0.542. Through the mathematical model-based cluster in structure software, all the accessions were divided into 8 subgroups and the information on the subgroup was used for the next association analysis. A high degree of LD was detected among syntenic markers, and the loci pairs with D'>0.5 accounting for 33.01% of the total. Twenty-eight markers were found to associate with the 5 traits, of which 7 marker associations were significantly different at P<0.01. Thirty-one marker-trait associations were detected herein; two for primordium period with an explanation of phenotypic variation (R2) range of 13.87%~17.41%; twelve for stipe diameter with a R2 range of 7.23%~23.02%; three for stipe length with a R2 range of 13.48%~23.55%; nine for pileus diameter with a R2 range of 5.11%~17.04%; five for yield per bottle with a R2 range of 9.47%~18.94%. The present study suggested that the genetic diversity and linkage disequilibrium of the tested F. velutipes germplasm were suitable for association analysis. The marker-trait association SSR primers developed in present study provide reference for the construction of germplasm for F. velutipes germplasm and molecular marker assisted breeding.
Key wordsFlammulina velutipes    Simple sequence repeat (SSR)    Population structure    Linkage disequilibrium (LD)    Association analysis
收稿日期: 2014-05-27      出版日期: 2015-01-06
基金资助:国家科技支撑项目“食用菌新品种培育及制种关键技术研究”
通讯作者: 尚晓冬     E-mail: xdshang@163.com
引用本文:   
陆欢 张丹 章炉军 王瑞娟 尚晓冬 谭琦. 金针菇种质资源5个农艺性状与SSR标记的关联分析[J]. , 2015, 23(1): 96-106.
链接本文:  
http://journal05.magtech.org.cn/Jwk_ny/CN/     或     http://journal05.magtech.org.cn/Jwk_ny/CN/Y2015/V23/I1/96
 
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