大口黑鲈载脂蛋白基因序列单核苷酸多态性及与生长性状相关分析

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摘要载脂蛋白(apoprotein, Apo)是血浆脂蛋白的重要组分，参与调节酶活性、脂肪运输与吸收和能量储存。为了探讨Apo基因多态性与大口黑鲈(Micropterus salmoides)生长性状的相关性，本研究根据大口黑鲈EST库中ApoA1、ApoA4和ApoC1基因设计引物扩增这3个基因的DNA片段，采用直接测序法和序列比对在ApoA1基因上筛选到1个颠换单核苷酸多态性(single nucleotide polymorphism, SNP)位点(A+489C)，在ApoC1上筛选到2个颠换SNP位点(A+24G和A+75C)，在ApoA4上筛选到1个转换SNP位点(A+633T)。利用SnaPshot分型技术对从同批次繁殖和同塘养殖的大口黑鲈群体中随机选取的159尾鱼中的4个SNPs位点进行基因型检测，统计分析显示，4个位点在所检测大口黑鲈群体中处于Hardy-Weinberg平衡状态，且ApoA4基因上的A+633T和ApoC1基因上的A+24G和A+75C位点在群体中的基因型和等位基因频率一致，3个位点完全连锁。运用软件Spss15.0中的一般线性模型对4个SNPs位点与大口黑鲈全长、体高和体质量进行关联分析，结果表明，ApoA1基因上的A+489C位点的3种不同基因型大口黑鲈在体质量和体高性状方面的差异显著(P＜0.05)；ApoA4基因上的A+633T位点和ApoC1基因上的A+24G和A+75C位点与生长性状的关联分析结果一致，不同基因型个体在全长、体高和体质量性状上的差异显著(P＜0.05)。A+489C、A+633T、A+24G和A+75C均与大口黑鲈生长性状显著相关，可作为大口黑鲈分子标记辅助选育研究的重要候选标记，应用于后续的分子辅助育种实践中。

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	李胜杰
	景燕娟
	白俊杰
	孙建国
	吴建开
	费志平

关键词 ：大口黑鲈，SNP，载脂蛋白，基因型，关联分析

Abstract：Abstract：In this study, the primers was designed according to cDNA sequences of apolipoprotein gene from EST database of largemouth bass and the genomic fragments of Apo-A1, Apo-A4 and Apo-C1 were amplified, respectively. 4 single nucleotide mutations were identified by using directly sequencing. The A+489C mutation was located in Apo-A1 gene. The A+633T mutation was located in Apo-A4 gene. The two mutations (A+24G and A+75C) were located in Apo-C1 gene. The genotype of each SNP in 159 largemouth bass was analyzed with the method of SnaPshot. Chi-square test showed that four SNP loci were in Hardy-Weinberg equilibrium state in the largemouth bass population. A+633T, A+24G and A+75C had the same genotype frequency, which formed two haplotypes (A and B) and constituted with three genotypes (AA, AB, and BB). This suggested that the three SNPs might completely link. A general linear model was used to analyze the relationship between polymorphisms of apolipoprotein gene and major growth traits. The result indicated that A+489C site showed significant association with body weight and body high (P<0.05). The different genotypes of A+633T in Apo-A4, A+24G and A+75C sites in Apo-C1 genes showed significant association with body weight, body high and total length, respectively (P<0.05). Our results showed that 4 SNPs had genetic effects on the growth traits in largemouth bass, which could be important candidate molecular markers for largemouth bass molecular marker-assisted selection.

Key words： Largemouth bass,SNP, apolipoprotein, genotype, correlation analysis

收稿日期: 2017-04-20 出版日期: 2018-01-01

基金资助:浙江省农业（水产）新品种选育重大科技专项;渔港建设和渔业产业发展专项;广东省科技计划项目;南京市高端人才团队引进计划项目

通讯作者: 白俊杰 E-mail: baijj2005@21cn.com;jjbai@163.net

引用本文:

李胜杰景燕娟白俊杰孙建国吴建开费志平. 大口黑鲈载脂蛋白基因序列单核苷酸多态性及与生长性状相关分析[J]. , 2018, 26(1): 132-139.

链接本文:

http://journal05.magtech.org.cn/Jwk_ny/CN/ 或 http://journal05.magtech.org.cn/Jwk_ny/CN/Y2018/V26/I1/132

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