Abstract:Abstract: A reference family was established by using three Large White boars (Sus scrofa )and seven Meishan dams(S.scrofa ). Blood samples of 140 F2 individuals were collected. Each sample was amplified by using primers of 7 microsatellites loci on chromosome 4 and 8 microsatellites loci on chromosome 7, and genotypes were identified. The linkage map of chromsome 4 and 7 was constructed by using CRIMAP software. In this population, average allele number was 3.6, average heterozygosity and polymorphic information content 0.531 and 0.463, average information meiosis 235. The genetic length of sex-average, female and male maps were 178.5, 197.7 and 172.2 cM for chromosome 4, and 179.1, 195.7 and 167.7 cM for chromosome 7 respectively. The average marker interval of chromosome 4 and 7 was 29.9 and 24.9 cM, respectively. Construction of linkage map will make the foundation for QTL mapping afterwards.
左 波 熊远著 苏玉虹 邓昌彦 余 雳 郑 嵘. 猪4号和7号染色体部分微卫星位点的遗传图谱构建[J]. , 2003, 11(1): 70-74.
Zuo Bo Xiong Yuanzhu Su Yuhong Deng Changyan Yu Li Zheng Rong. Genetic Linkage Map Construction of Some Microsatellites on Pig Chromosome 4 and 7. , 2003, 11(1): 70-74.