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2025年8月8日 星期五
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用水稻基因芯片筛选小麦耐旱相关基因的研究
刘国振
河北农业大学生命科学学院
Identification of wheat drought-tolerant gene using rice microarray
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摘要 小麦耐旱机理的研究具有重要意义,为了了解小麦在干旱逆境条件下的基因转录规律,本研究采用PEG6000对耐旱小麦品种旱选10号进行拟旱处理,分别提取0、1、6和24小时植株的总RNA,经反转录荧光标记制备cDNA探针,并将其与水稻全基因组芯片进行杂交,扫描采集数据后并进行分析。结果表明,随着处理时间的延长,差异表达基因的数量增加,对差异基因进行功能分类,能量代谢途径相关基因所占比例随处理时间的延长而逐渐增加,大部分为光合作用相关基因,其总体表达趋势为上调,但Psbr和Rubisco编码基因的转录水平为下调,暗示它们在耐旱反应中发挥着一定作用。
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刘国振
关键词 小麦耐旱基因芯片转录    
Abstract:It is important to understand the mechanism of wheat tolerance to drought, to investigate the wheat transcriptional profiling drought stress, a drought tolerance variety-Hanxuan 10 was treated by PEG6000,RNA samples were collected at 0, 1, 6, and 24 hours respectively, influence dye-labeled complimentary DNA were used to hybridize with the whole genome rice gene chip, which contains over 60K oligos designed based on rice sequence. Data analysis indicated that the number of differentially expressed genes was increased as longer PEG treatment, functional category analysis results indicate that number of energy metabolism pathway-related gene was increased and account for more portion of differentially expressed genes. Most of the photosynthesis related genes was up-regulated but interestingly, Psbr and Rubisco coding genes were down-regulated, suggesting their potential role in drought tolerance response.
Key wordsWheat    Drought tolerance    microarray    transcription
收稿日期: 2007-01-30     
通讯作者: 刘国振   
引用本文:   
刘国振. 用水稻基因芯片筛选小麦耐旱相关基因的研究[J]. , 2007, 15(5): 0-.
链接本文:  
http://journal05.magtech.org.cn/Jwk_ny/CN/      或     http://journal05.magtech.org.cn/Jwk_ny/CN/Y2007/V15/I5/0
 
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