Abstract：With the Overlap-PCR methods, the 1 278 bp cds sequences of HCRTR1 gene in Qinchuan cattle was got. The fragments were cloned into the pMD18-T vector and the positive clone plasmids was sequenced. Results showed that the cloned fragments had 98% homology with the sequence of GenBank after being blasted. Digested the plasmid with BamHⅠand HindⅢ and the 1 278 bp was obtained. The obtained fragment was ligated with the longer fragment digested with the same restriction enzyme from the expression vector, pET32 a(+). The positive recombinated plasmid was transformed Bl21(DE3) and induced with IPTG. But the HCRTR1 gene cannot be expressed by E.coli. And the tranmembrane structure may explain why.
张丽 张良志 陈宏 张爱玲 张琴. Overlap-PCR克隆秦川牛HCRTR1基因及其在大肠杆菌中的表达分析[J]. , 2008, 16(4): 0-.
. The CDS Cloning of HCRTR1 Gene with Over-lap PCR from Qinchuan Cattle and its Expression in E.coli. , 2008, 16(4): 0-.
