Secretion System Composition, Protein Interaction Analysis and Effector Proteins Prediction of Pleurotus eryngii Soft Rot Pathogen
GU Tong-Tong1,2, ZHAO Shuang1,2, SONG Shuang1,2, LIU Yu1,2, RONG Cheng-Bo1,2*
1 Institute of Plant Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China;
2 Beijing Engineering Research Center for Edible Mushroom, Beijing 100097, China
Abstract：Soft rot disease which is caused by Erwinia beijingensis is the major disease in Pleurotus eryngii. Until now, the pathogenic mechanism of E. beijingensis is unknown. In the present study, the whole genome information of E. beijingensis was obtained by Nanopore and Illumina platform, the gene function annotation and secretion system composition were analyzed. The protein interactions of secretion system were predicted by string website (https://www. string-db. org/), and the effector proteins were predicted by SecRet6 and effectvet3 software. The genome size of E. beijingensis was 4.11 Mb, with GC% of 50.17%, encoding 3 815 genes. E. beijingensis displayed type Ⅰ, type Ⅲ, type Ⅳ and type Ⅵ secretion system (T1SS, T3SS, T4SS and T6SS). T1SS was composed of 3 proteins, including inter membrane (IM) component, membrane fusion protein (MFP) and outer membrane (OM) protein channel, among which 2 pairs of proteins have interactions. It is speculated that 36 proteins were secreted through T1SS. T3SS consisted of 9 Hrc proteins and 11 Hrp proteins, with 88 pairs of protein interactions, and 154 effector proteins were predicted to be secreted by the T3SS. T4SS consisted of 9 VirB proteins with 21 pairs of protein interactions and 11 effector proteins. E.beijingensis had 2 T6SS, T6SS-1 was composed of 13 T6SS proteins and 11 hypothetical proteins, 104 protein interactions were formed between T6SS proteins. T6SS-2 included 11 T6SS proteins and 8 hypothetical proteins, 55 pairs of protein interactions were found in T6SS-2. It was predicted that 6 effector proteins secreted into host cell by T6SS. 58 secretion system genes could be expressed during infection, and 34 genes showed significant different expression. This study analyzed the composition, protein interactions and effector proteins of secretion systems in E. beijingensis from the genomic level, which provided basic data for the analysis of pathogenic genes and theoretical basis for further analysis of pathogenic mechanism.
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