Correlation Analysis of SNP and Haplotypes of SCD Gene with Growth Traits in Qinghai Plateau Yak (Bos grunniens)
QI Zeng-Yuan1, GAO Zhan-Hong2, ZHOU Jian-Qiang1, HAN Yin-Cang2, LIU Xiu1*, SUN Yong-Gang2*
1 Gansu Key Laboratory of Herbivore Biotechnology, College of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, China;
2 Qinghai Academy of Animal Science and Veterinary Medicine, Xining 810016, China
Abstract：Stearyl-CoA desaturase (SCD) is the key enzyme of monounsaturated fatty acid biosynthesis, which plays an important role in the lipid synthesis and oxidation dynamic balance. To explore the qinghai plateau yak SCD gene polymorphism and its correlation with growth traits, DNA sequencing method was used for gene polymorphism analysis,linkage disequilibrium analysis and the method of general linear model were used for correlation analysis. The results showed that there were 2 SNPs in SCD gene (g. 6614C>A and g.6660C>T), there were 2 genotypes in g.6614C>A and 3 genotypes in g.6660C>T. χ2 test showed that the g.6614C>A balanced in the Hardy Weinberg lattice state, g.6660C>T was in a state of imbalance. g.6614C> A was low polymorphic (PIC<0.25), and g.6660C>T was moderately polymorphic (0.50>PIC>0.25). And growth character correlation analysis found that the body weight, body height, body oblique length and tube circumference of CA genotype at g.6614C>A locus were significantly higher than those of CC genotype (P<0.05), but there was no significant difference in chest circumference between CA genotype and CC genotype. g. 6660C>T site of TT genotype individuals had no significant difference with CT and CC genotype, individuals weight, body height and chest circumference of TT genotype were significantly higher than CC and CT genotype (P<0.05), while TT genotype tube circumference was extremely significantly higher than CC genotype (P<0.01). Haplotype and linkage disequilibrium analysis showed that there were 4 haplotypes in the 2 loci, and there was no strong linkage between the 2 loci (r2=0.027). The combined haplotype analysis showed that H1H3 haploid type was the optimal combination. In conclusion, SCD gene polymorphism was related to some growth traits of Qinghai plateau yak, which can be used as candidate genes for marker-assisted selection in yaks. This study provides reference for improving yak breeding.
祁增源, 高占红, 周建强, 韩银仓, 刘秀, 孙永刚. 青海高原型牦牛 SCD 基因 SNP 及单倍型与生长性状的关联性分析[J]. 农业生物技术学报, 2022, 30(7): 1314-1320.
QI Zeng-Yuan, GAO Zhan-Hong, ZHOU Jian-Qiang, HAN Yin-Cang, LIU Xiu, SUN Yong-Gang. Correlation Analysis of SNP and Haplotypes of SCD Gene with Growth Traits in Qinghai Plateau Yak (Bos grunniens). 农业生物技术学报, 2022, 30(7): 1314-1320.
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