Effects of Long-term Biogas Slurry Fertilization on Bacterial Community Structure and Diversity in Soils of Areca Taro (Colocasia esculenta)
LIU Lan-Ying, HUANG Wei, LI Ying, LYU Xin, CHEN Li-Hua, LI Yue-Ren*
Institute of Agricultural Quality Standards and Testing Technology Research, Fujian Academy of Agricultural Science/Fujian Key Laboratory of Agro-products Quality & Safety, Fuzhou 350003, China
Abstract：Biogas slurry irrigation in farmland can solve the problem of subsequent disposal and the reduction of chemical fertilizers, which has become one of the important ways of resource utilization of livestock wastewater. In this study, a 6-year field experiment was conducted around a pig farm in Jian'ou City, Fujian Province to investigate the effects of biogas slurry application on soil bacterial community of areca taro (Colocasia esculenta) in different seasons and depths. Soil samples were collected in summer and winter at two different depths (0~20 cm and 20~40 cm). The soil with no application of biogas slurry was also collected as the control group. Bacterial community composition and diversity were investigated using Illumina MiSeq sequencing technology, and its relationship with soil properties was discussed. The results showed that the contents of soil organic matter, alkali-hydrolyzable nitrogen and available potassium increased in the biogas slurry treatment over control treatment. Also the contents of them were generally higher at the 0~20 cm than that at the 20~40 cm soil layers. Compared with the control, the long-term application of biogas slurry had a positive effect on improving the bacterial richness. For bacterial diversity, it varied according to seasons. The richness and diversity of soil bacteria in different seasons basically decreased with increasing soil depth, and the application of biogas slurry changed their seasonal variation. Bacterial community structure in the soil varied with biogas slurry irrigation. Proteobacteria, Actinobacteria, Firmicutes were the dominant plyumn of all soil samples. Long-term application of biogas slurry generally increased the relative abundance of Proteobacteria, Planctomycetes and Nitrospirae, but it decreased Actinobacteria. Moreover, Massilia and Geobacter belonging to Proteobacteria increased significantly in the long-term biogas slurry applied soil (P<0.05), while the proportion of Mizugakiibacter showed an opposite trend. Through redundancy analysis, it was found that soil bacterial community structure was greatly affected by soil available potassium. Long-term application of biogas slurry caused changes in soil microenvironment in different seasons and depths, and then led to changes in community composition and diversity of soil bacteria. These results can serve as a theoretical basis for evaluating the effects of biogas slurry application on the soil micro-ecological environment.
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