Effects of Lactic Acid Bacteria on Intestinal Microflora and Growth Performance of Hybrid Snakehead (Channa argus (♀) × Channa maculata (♂))
OU Mi1, XU Sheng-Yun1,2, CHEN Kun-Ci1,2, LUO Qing1, LIU Hai-Yang1, LIANG Xin-Qiu1, ZHAO Jian1,2,*
1 Key Laboratory of Tropical and Subtropical Fishery Resources Application and Cultivation, Ministry of Agriculture and Rural Affairs, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510380, China; 2 College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China
Abstract：Channa are economically important freshwater fish, high-density and intensive culture have led to the deterioration of its breeding environment and frequent diseases, which greatly hinders the development of snakehead breeding industry. To study the effects of lactic acid bacteria (LAB) on growth performance and intestinal microflora of hybrid snakehead (Channa argus (♀) × Channa maculata (♂)), the fish in experimental group (QXSY group) were fed in the basal diet supplemented with LAB, while those in control group (DZ group) were fed in the basal diet. The intestines and their contents were collected from DZ and QXSY groups on 0, 26, 60 and 73 d, respectively, for high-throughput sequencing to detect the intestinal microbial structure. At the same time, 90 fish were randomly sampled from each group to measure body length and weight at each sampling, and the feed consumption, morbidity and the rate of surviving fish were recorded. The results showed that the diversity of intestinal microflora in hybrid snakehead could be changed by supplementing LAB The diversity of intestinal microflora in QXSY3 group (the experimental group was sampled on the 60th day) was higher than that in DZ3 group (the control group was sampled on the 60th day), while there was no significant difference in Lactobacillus content between the two groups. Lactobacillus content in QXSY4 group the experimental group was sampled on the 73rd day) was much higher than that in DZ4 group (the control group was sampled on the 73rd day), at the same time, the diversity of intestinal microflora in QXSY4 group was lower than that in DZ4 group, indicating that LAB 'settled' and became the dominant microflora. Some bacterial communities also changed with supplement of LAB, Cetobacterium and Lactobacillus in DZ and QXSY group showed an opposite trend. At the end of the experiment, the growth rate of QXSY group was higher than that of DZ group by (12.0±2.3)%, and the feed coefficient of QXSY group (1.02) was lower than that of DZ group (1.13). The results showed that LAB in the feed of hybrid snakehead could improve the community structure of intestinal bacterial, increase the number of potential probiotics, reduce the number of potential harmful bacteria, optimize the intestinal environment of hybrid snakehead. This research provides a theoretical basis for the application of LAB in the healthy breeding of hybrid snakehead.
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