The Expression of Genes Related to Clathrin-mediated Endocytosis in Estrus Ovary of Congjiang Xiang Pig (Sus scrofa)
TANG Liang-Ting1,2, HUANG Shi-Hui1, WANG Jia-Fu1,*, RAN Xue-Qin1,*, LI Sheng1, NIU Xi1
1 Institute of Agri-bioengineering / College of Animal Science, Guizhou University, Guiyang 550025, China; 2 Guizhou Open University, Guiyang 550023, China
Abstract：During the development of ovarian follicles, gap junctions between oocytes and surrounding granulosa cells mediate intercellular communication and material transport. Gap junction internalization refers to the clathrin-mediated endocytosis (CME) process. In order to study the effect of CME-related genes on ovary function of Congjiang Xiang pig (Sus scrofa), transcriptome sequencing technology was used to analyze the expression of CME-related genes and the alternative splicing of transcripts in Congjiang Xiang pigs ovaries at estrus stage by Limma, DESeq2, rMATs softwares and reverse transcription PCR (RT-PCR) method. Transcriptome changes at diestrus stage were used as controls. As a result, 8 CME genes were detected in the ovaries of Congjiang Xiang pigs, including DAB adaptor protein 2 (DAB2), adaptor related protein complex 2 subunit μ1 (AP2M1), clathrin light chain B (CLTB), clathrin light chain A (CLTA), dynamin-1 (DNM1), myosin Ⅵ (MYO6), dynamin-2 (DNM2) and clathrin heavy chain (CLTC). Among them, the differentially expressed genes were DAB2, AP2M1 and CLTB. Except for CLTB, the other 7 genes produced different alternative splicing events between estrous and diestrous ovaries, and the 7 transcripts produced by these alternative splicing events were the new transcripts not included in the Ensembl database up to now. It showed that alternative splicing was an important regulation mode of ovary in Congjiang Xiang pigs at estrus stage, and a specific network of protein interaction was formed by producing new transcripts and proteins during CME. The results of this study indicated that the internalization of gap junction was regulated by the expression of CME-related genes and splicing of transcripts at transcription and post-transcription levels, thus regulating the ovarian function of Congjiang Xiang pig. These researches would help to understand of the post-transcriptional regulation process and the molecular regulation mechanism of porcine ovarian function.
唐靓婷, 黄世会, 王嘉福, 冉雪琴, 李升, 牛熙. 从江香猪发情期卵巢网格蛋白介导的内吞作用相关基因表达研究[J]. 农业生物技术学报, 2021, 29(9): 1741-1751.
TANG Liang-Ting, HUANG Shi-Hui, WANG Jia-Fu, RAN Xue-Qin, LI Sheng, NIU Xi. The Expression of Genes Related to Clathrin-mediated Endocytosis in Estrus Ovary of Congjiang Xiang Pig (Sus scrofa). 农业生物技术学报, 2021, 29(9): 1741-1751.
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