Abstract:Wool fiber is one of the important economic products of sheep. The development of the wool market has stimulated people's demand for different characteristics of wool. Peratin-associated proteins (KAPs)genes--KRTAPs and keratin-associated proteins (KIFs) genes and their respective variant genes are candidate genes for identifying the genetic basis of wool fiber differences. Using the homologous genes of human or goat and other mammalian keratins, we can identify the sheep genome and explore new potential functional KRTAPs or KIFs genes. In this review, the morphological structure of hair follicles is introduced, and the development of keratin-related protein genes is reviewed. The expression and location of KAP protein in hair follicles are also described. Finally, the effect of KRTAPs gene polymorphism on wool traits is summarized. This review provides a theoretical basis for the molecular breeding of sheep hair follicle growth and development and hair fiber traits.
和东迁, 陶金忠, 陈丽尧, 王俊奎. 与毛纤维性状相关的角蛋白相关蛋白研究进展[J]. 农业生物技术学报, 2021, 29(6): 1198-1205.
HE Dong-Qian, TAO Jin-Zhong, CHEN Li-Yao, WANG Jun-Kui. Research Progress of Keratin-related Proteins Related to Hair Fiber Traits. 农业生物技术学报, 2021, 29(6): 1198-1205.
[1] 阿布来提·苏来曼, 田月珍, 许琴, 等. 2017. 苏博美利奴羊胎儿皮肤毛囊结构及形态发育[J]. 中国农业科学, 50(16): 3226-3235. (Abulaiti S L M, Tian Y Z, Xu Q, et al.2017. The structure and morphological development of fetal skin and hair follicles of Submerino sheep[J]. China Agricultural Sciences, 50(16): 3226-3235.) [2] 金梅, 王瑞, 王婧, 等. 2017. HGT KAPs基因家族在辽宁绒山羊皮肤毛囊中的表达研究[J]. 中国畜牧杂志, 53(2): 40-46. (Jin M, Wang R, Wang J, et al.2017. Expression of HGT KAPs gene family in skin hair follicles of Liaoning cashmere goats[J]. Chinese Journal of Animal Husbandry, 53(2): 40-46.) [3] 李文浩. 2019. 绵羊角蛋白基因鉴定及毛用性状关联性研究[D].硕士学位论文, 甘肃农业大学, 导师: 罗玉柱, pp. 55-75. (Li W H, 2019, Sheep keratin gene identification and correlation study of wool traits[D]. Thesis for M.S., Gansu Agricultural University, Supervisor: Luo Z Z. pp. 55-75.) [4] 杨万宝. 2016. 绵羊毛囊皮质部基因KAP13-1对毛纤维组装调控的研究[D]. 硕士学位论文, 华中农业大学, 导师: 余梅, pp. 36-55. (Yang W B, 2016. Study on the regulation of sheep hair follicle cortex gene KAP13-1 on hair fib;er assembly[D]. Thesis for M.S., Huazhong Agricultural University, Supervisor: Yu M, pp. 36-55.) [5] Bai L, Wang J, Zhou H, et al.2019. Identification of ovine KRTAP28-1 and its association with wool fibre diameter[J]. Animals. 9(4): 142. [6] Bai L, Gong H, Zhou H, et al.2018, A nucleotide substitution in the ovine KAP20-2 gene leads to a premature stop codon that affects wool fibre curvature[J]. Animal Genetics, 49(4): 357-358. [7] Beh K J, Callajhan M J, Leish Z, et al.2001. A genome scan for QTL affecting fleece and wool traits in Merino sheep[J]. Wool Technology & Sheep Breeding, 2(49): 88-97. [8] Dongald F O, Joy L W, Stephen L R, et al.1984. Cortical cell types and their distribution in wool fibres[J]. Australian Journal of Biological Sciences, 37: 237-55. [9] Frenkel M J, Powell B C, Ward K A, et al.1989. The keratin BIIIB gene family: Isolation of cDNA clones and structure of a gene and a related pseudogene[J]. Genomics, 4(2): 182-191. [10] Gillespie J M, Darskus R L, 1971. Relation between the tyrosine content of various wools and their content of a class of proteins rich in tyrosine and glycine[J]. Australian Journal of Biological Sciences, 24: 1189-1197. [11] George E R, 2004. Hair follicle differentiation and regulation[J]. International Journal of Developmental Biology, 48(2-3): 163. [12] Gong H, Zhou H, Mckenzie G W, et al., 2012. An updated nomenclature for keratin-associated proteins (KAPs)[J]. International Journal of Biological Sciences, 8(2): 258. [13] Gong H, Zhou H, Yu Z, et al.2011a. Identification of the ovine keratin-associated protein KAP1-2 gene (KRTAP1-2)[J]. Experimental Dermatology, 20(10): 815-819. [14] Gong H, Zhou H, Hickford J G H.2010. Polymorphism of the ovine keratin-associated protein 1-4 gene (KRTAP1-4)[J]. Molecular Biology Reports, 37(7): 3377-3380. [15] Gong H, Zhou H, Dyer J M, et al.2011b. Identification of the ovine KAP11-1 gene (KRTAP11-1) and genetic variation in its coding sequence[J]. Molecular Biology Reports, 38(8): 5429-5433. [16] Gong H, Zhou H, Dyer J, et al.2011c. Identification of the keratin-associated protein 13-3 (KAP13-3) gene in sheep[J]. Open Journal of Genetics, 1(3): 60-64. [17] Gong H, Zhou H, Dyer J M, et al.2014. The sheep KAP8-2 gene, a new KAP8 family member that is absent in humans[J]. SpringerPlus, 3(1): 1-5. [18] Gong H, Zhou H, Wang J, et al.2019. Characterisation of an ovine keratin associated protein (KAP) gene,which would produce a protein rich in glycine and tyrosine, but lacking in cysteine[J]. Genes, 10(11): 848. [19] Gong H, Zhou H, Bai L, et al.2018. Associations between variation in the ovine high glycine-tyrosine keratin-associated protein gene KRTAP20-1 and wool traits[J]. Journal of Animal Science, 97(2): 587-595. [20] Harland D P,Vernon J A, Woods J L, et al.2018. Intrinsic curvature in wool fibres is determined by the relative length of orthocortical and paracortical cells[J]. The Journal of Experimental Biology, 221(6): jeb172312. [21] He, D Q, Chen L Y, Luo, F, et al.2021. Differentially phosphorylated proteins in the crimped and straight wool of Chinese Tan sheep[J]. Journal of Proteomics. 235. 104115. [22] Jenkins B J, Powell B C, 1994. Differential expression of genes encoding a cysteine-rich keratin family in the hair cuticle[J]. Journal of Investigative Dermatology, 103(3): 310-317. [23] Jolles P, Zahn H, Hocker H.2001, Formation and Structure of Human Hair[M]. Birkhäuser, ISBN:978-3764351199, pp. 7-16. [24] Koehn H, Plowman J E, Morton J D, et al.2015. Identification and quantitation of major structural proteins from enriched cuticle fractions of wools of different breeds[J]. New Zealand Journal of Agricultural Research, 58(4): 1-9. [25] Liu Y, Wang L, Li X, et al.2017. High-throughput sequencing of hair follicle development-related micrornas in cashmere goat at various fetal periods[J]. Saudi Journal of Biological Sciences, 1494-1508. [26] Li S, Zhou H, Gong H, et al.2017a. Identification of the ovine keratin-associated protein 22-1 (KAP22-1) gene and its effect on wool traits[J]. Genes, 8(1): 27. [27] Li S, Zhou H, Gong H, et al.2017b. Identification of the ovine keratin-associated protein 26-1 gene and its association with variation in wool traits[J]. Genes, 8(9): 225. [28] Li S, Zhou H, Gong H, et al.2019a. Identification of the ovine keratin-associated protein 21-1 gene and its association with variation in wool traits[J]. Animals (Basel), 9(7): 450. [29] Li S, Zhou H, Gong H, et al.2020. The mean staple length of wool fibre is associated with variation in the ovine keratin-associated protein 21-2 gene[J]. Genes, 11(2): 148. [30] Li S W, Ouyang H S, Rogers G E, et al.2009. Characterization of the structural and molecular defects in fibres and follicles of the merino felting lustre mutant[J]. Experimental Dermatology, 18(2): 134-142. [31] Li W, Gong H, Zhou H, et al., 2019b, Variation in KRTAP6-1 affects wool fibre diameter in New Zealand Romney ewes[J]. Archives Animal Breeding, 62(2): 509-515. [32] Liu Y X, Shi G Q, Wang H X, et al.2014. Polymorphisms of KAP6, KAP7, and KAP8 genes in four Chinese sheep breeds[J]. Genetics and Molecular Research: GMR, 13(2): 3438-3445. [33] Liu G B, Liu R Z, Tang X H, et al.2015. Expression profiling reveals genes involved in the regulation of wool follicle bulb regression and regeneration in sheep[J]. International Journal of Molecular Sciences, 16: 9152-9166. [34] Mclaren R J, Rogers G R, Davies K P, et al.1997. Linkage mapping of wool keratin and keratin-associated protein genes in sheep[J]. Mammalian Genome, 8(12): 938-940. [35] Parsons Y M, Cooper D W, Piper L R, et al.1994. Evidence of linkage between high-glycine-tyrosine keratin gene loci and wool fibre diameter in a Merino half-sib family[J]. Animal Genetics. 25: 105-108. [36] Plowman J E, Harland D P, Ganeshan S, et al.2015. The proteomics of wool fibre morphogenesis[J]. Journal of Structural Biology, 191(3): 341-351. [37] Plowman J, Thomas A, Perloiro T, et al.2019. Characterisation of white and black merino wools: A proteomics study[J]. Animal, 13(3): 659-665. [38] Powell B C, Antonietta N, 2010. Regulation of Keratin Gene Expression in Hair Follicle Differentiationa. //The Molecular and Structural Biology of Hair[M]. Annals of the New York Academy of Sciences 642. pp, 1-20. [39] Powell B C, Nesci A, Rogers G E, 2010. Regulation of Keratin Gene Expression in Hair Follicle Differentiationa[J]. Annals of the New York Academy of ences, 642(The Molecular and Structural Biology of Hair):1-20. [40] Powell B C, Rogers G E.1997. The role of keratin proteins and their genes in the growth, structure and properties of hair[J]. EXS, 78: 59-148. [41] Rogers M A, Langbein L, Praetze W S, et al.2006. Human hair keratin-associated proteins (KAPs)[J]. International Review of Cytology. 251: 209. [42] Sawant M S, Leube R E, 2016. Consequences of keratin phosphorylation for cytoskeletal organization and epithelial functions[J]. International Review of Cell & Molecular Biology, 330: 171. [43] Sallam A M, Gad-Allah A A, Al-Bitar E M, 2020. Association analysis of the ovine KAP6-1 gene and wool traits in Barki sheep[J]. Animal Biotechnology, DOI: 10.1080/10495398.2020.1749064. [44] Swart L S, Haylett T, 1971. Studies on the high-sulphur proteins of reduced merino wool. Amino acid sequence of protein SCMKB-3B 4[J]. Biochemical Journal, 123(2): 201-210. [45] Tao J, Zhou H, Gong H, et al.2017a. Variation in the KAP6-1 gene in Chinese Tan sheep and associations with variation in wool traits[J]. Small Ruminant Research, 154: 129-132. [46] Tao J, Zhou H, Yang Z, et al.2017b. Variation in the KAP8-2 gene affects wool crimp and growth in Chinese Tan sheep[J]. Small Ruminant Research, 149: 77-80. [47] Ullah F, Jamal S M, Ekegbu U J, et al.2019. Polymorphism in the ovine keratin-associated protein gene KRTAP7-1 and its association with wool characteristics[J]. Journal of animal science, 98(1): 1-7. [48] Wang J, Che L, Hickford J, et al.2017a. Identification of the caprine keratin-associated protein 20-2 (KAP20-2) gene and its effect on cashmere traits[J]. Genes, 8(11): 328. [49] Wang J, Zhou H, Zhu J, et al.2017b. Identification of the ovine keratin-associated protein 15-1 gene (KRTAP15-1) and genetic variation in its coding sequence[J]. Small Ruminant Research, 153: 131-136. [50] Wilding E.2016. Analysis of the keratin associated protein gene KRTAP6-1 in New Zealand Wiltshire sheep[D]. Lincoln University, Theis for BA, Supervisor: Hickford J, pp, 16-18. [51] Yu Z, Plowman J E, Maclean P.2018. Ovine keratome identification, localisation and genomic organisation of keratin and keratin-associated protein[J]. Journal of Chemical Ecology, 639: 1-8. [52] Yu Z, Gordon S W, Nixon A J, et al.2009. Expression patterns of keratin intermediate filament and keratin associated protein genes in wool follicles[J]. Differentiation, 77(3): 307-316. [53] Zhao Z C, Liu G B, Li X Y, et al.2016. Characterization of the promoter regions of two sheep keratin-associated protein genes for hair cortex-Specific expression[J]. PLOS ONE, 11(4): e0153936. [54] Zhou H, Gong H, Li S, et al., 2015. A 57-bp deletion in the ovine KAP6-1 gene affects wool fibre diameter[J]. Journal of Animal Breeding and Genetics, 132(4): 301-307. [55] Zhou H, Gong H, Wang J, et al.2016. Identification of four new gene members of the KAP6 gene family in sheep[J]. Scientific Reports, 6(1): 24074. [56] Zhou H, Gong H, Yan W, et al.2012. Identification and sequence analysis of the keratin-associated protein 24-1 (KAP24-1) gene homologue in sheep[J]. Gene, 511(1): 62-65.