Correlation Analysis of CMTM2 Gene Polymorphism with Growth Traits in Inner Mongolia Cashmere Goats (Capra hircus)
WANG Cong-Liang1,*, LI He-Lin1,*, XU Zheng-Yu1, QU Cheng1, BAI Jing-Jing1, SHI Lei1, SONG Xiao-Yue1, LI Long-Ping1, QU Lei1,**, ZHU Hai-Jing1,2,3,**
1 Life Science Research Center, Yulin University, Yulin 719000, China; 2 Shaanxi Province 'Four Subjects and One Joint' Sheep and Goat Engineering & Technology University & Enterprise Alliance Research Center, Shenmu 719318, China; 3 Shaanxi Haoli Cashmere Goat Technology Development Co., Ltd., Zizhou 718499, China
Abstract：Chemokine-like factor (CKLF) superfamily member 2 (CKLFSF2), named as CKLF-like MARVEL transmembrane domain-containing 2 (CMTM2), plays an important role in spermatogenesis, bone formation and body development. Therefore, it is speculated that the CMTM2 gene might also play an important role in normal growth and development of animals. This study explored CMTM2 genetic polymorphisms and its correlation with growth traits in Inner Mongolia cashmere goats (Capra hircus), and obtained the growing-related genetic markers, hoping to accelerate the breeding process of Inner Mongolia cashmere goat and superfine cashmere goats. In this study, Inner Mongolia cashmere goat (including 460 yearling sheep and 215 adult sheep) were selected as the research objects, and genome DNA was extracted from ear tissues to perform PCR amplification for the detection of insertion/deletion (InDel) mutation of CMTM2 gene, and the correlation between the variations and growth traits was analyzed. The results showed that there was a 14-bp InDel mutation in the CMTM2 gene promoter region of Inner Mongolian cashmere yearling and adult goats, there were 2 types of alleles I and D, and the allele frequency of I was higher than that of D in both yearlinging and adult goats. Homozygous insertion type (I), heterozygous type (ID) and homozygous deletion type (DD) were produced in the mutation. The analysis of polymorphic information content (PIC) indicated that the site was moderately polymorphic in both yearling and adult goats (0.25<PIC<0.50), and this variation did not conform to Hardy-Weinberg equilibrium in the yearling goat population (P<0.05). Further correlation analysis showed that in the yearling goats (n=460), the mutation was significantly correlated with height at hip cross (P<0.05). In the adult goat population (n=215), the mutation site was significantly correlated with body height (P<0.05), heart girth (P<0.05), height at hip cross (P<0.05), chest depth (P<0.05), and fluff fineness (P<0.05). In all of the goat population (n=675), the mutation site was significantly correlated with fluff fineness as well (P<0.05). The above results suggested that the 14-bp InDel within CMTM2 gene promoter region had significant or extremely significant correlation with some growth traits of Inner Mongolia cashmere goats, and different analysis methods showed that the mutation was significantly associated with fluff fineness of goats. Therefore, CMTM2 gene could be used as a candidate gene for the selection of growth traits in Inner Mongolia cashmere goats. The present study provides reference for the selection of Inner Mongolia cashmere goats and superfine cashmere goats.
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