Genome-wide Association Study on Plant Height of Wheat (Triticum aestivum) from Yellow and Huai Valley Wheat Region of China Under Different Nitrogen Environments
ZHANG Na1, ZHANG Xi-Lan2, ZHAO Ming-Hui3, QIAO Wen-Chen3, FU Xiao-Yi4, HE Ming-Qi4, SUN Li-Jing5, LI Hui5, JI Jun2,*
1 Jiangsu Xuhuai Regional Institute of Agricultural Sciences, Xuzhou 221131, China; 2 Center for Agricultural Resources Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Shijiazhuang 050021, China; 3 Institute of Dry Farming Agriculture, Hebei Academy of Agricultural and Forestry Sciences, Hengshui 053000, China; 4 Shijiazhuang Academy of Agricultural and Forestry Sciences, Shijiazhuang 050041, China; 5 Institute of Cereal and Oil Crops, Hebei Academy of Agricultural and Forestry Sciences, Shijiazhuang 050035, China
Abstract：Plant height (PH) is an important agronomic trait of wheat (Triticum aestivum), which is closely related to high and stable yield. Nitrogen is an essential mineral nutrient for plant growth and development, critically affects PH and yield of wheat. The sensitivity to nitrogen varies among different cultivars. In order to explore genetic loci influencing PH and PH tolerance to low nitrogen stress, and to provide excellent genetic resources of dwarf and high nitrogen efficiency, in this study, 132 elite wheat varieties (lines) mainly from the north Yellow and Huai Valley Wheat Region of China in recent 30 years were selected to carry out genome-wide association study (GWAS). PH and tolerant index (TI) of PH to low nitrogen stress under 8 environments with different nitrogen fertilizer conditions were identified. The genetic data were acquired by Affymetrix Wheat 55K SNP chip. Finally, 13 SNPs distributed on 1B, 1D, 2A, 2B, 2D, 3B, 4D, 5A, 6B and 7D were identified to be significantly associated with PH in 3 or more environments, which could explain 9.6%~26.6% of the phenotypic variation. Among them 4 significant association SNPs on 1B (2), 3B and 7D could be identified in both low nitrogen environment (LN) and high nitrogen environment (HN). AX-108824683-7D was significantly associated with PH in 7 environments, accounting for 16.8%~26.6% of the phenotypic variation. This locus has not been reported in previous studies, suggesting that it is a new dwarf gene locus. 7 SNPs were identified to be significantly associated with tolerant index of PH to low nitrogen stress, among which AX-111004994-4B was stably identified in 2 environments, explained 12.4%~13.1% of the phenotypic variation. The results provide references for dwarfing and high nitrogen efficiency in wheat molecular breeding.
张娜, 张希兰, 赵明辉, 乔文臣, 傅晓艺, 何明琦, 孙丽静, 李辉, 纪军. 不同氮素条件下黄淮麦区小麦株高全基因组关联分析[J]. 农业生物技术学报, 2021, 29(2): 207-215.
ZHANG Na, ZHANG Xi-Lan, ZHAO Ming-Hui, QIAO Wen-Chen, FU Xiao-Yi, HE Ming-Qi, SUN Li-Jing, LI Hui, JI Jun. Genome-wide Association Study on Plant Height of Wheat (Triticum aestivum) from Yellow and Huai Valley Wheat Region of China Under Different Nitrogen Environments. 农业生物技术学报, 2021, 29(2): 207-215.
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