Study on Differential Tissue Expression of MEF2C Splice Variants in Xingyi Duck (Anas platyrhynchos)
YE Tao1,2,3, YANG Hua-Ting1,2,3, LI Hui1,2,3,*, LUO Wei1,2,3, XIAO Tao1,2,3, LI Xing-Cai1,2,3, WU Gu-Rong4
1 Key Laboratory of Animal Genetics, Breeding and Reproduction in the Plateau Mountainous Region, Ministry of Education, Guizhou University, Guiyang 550025, China; 2 Guizhou Provincial Key Laboratory of Animal Genetics, Breeding and Reproduction, Guizhou University, Guiyang 550025, China; 3 College of Animal Science, Guizhou University, Guiyang 550025, China; 4 Agriculture and Rural Affairs Bureau of Xingyi City, Qiandongnan Prefecture 562400, China
Abstract：At present, the expression of splice variants of myocyte enhancer factor 2C gene (MEF2C) in ducks (Anas platyrhynchos) has not been deeply studied. In order to understand the expression difference of MEF2C variants in tissues, RT-PCR was used to clone the full-length CDS of MEF2C gene in Xingyi duck. The results showed that 2 splice variants of MEF2C-a (GenBank No. MZ497418) and MEF2C-b (GenBank No. MZ497419) were successfully cloned, and the encoded amino acid sequences shared more than 95% homology with pigeon (Columba livia), quail (Coturnix japonica) and chicken (Gallus gallus), which indicated that MEF2C gene was highly conserved in the evolution process. The results of qPCR showed that MEF2C-a and MEF2C-b were expressed in both male and female ducks, and highly expressed in brain, myocardium, leg muscle and chest muscle. The expression patterns of the 2 splice variants in female and male ducks were different. The expression level of MEF2C-a in most tissues of male ducks was higher than that of MEF2C-b, the opposite in female ducks. The expression of 2 splice variants in leg muscles and chest muscles did not increase with increasing age. The present results provide basic materials for further study on the mechanism of MEF2C splice variants in duck muscle development.
叶涛, 杨华婷, 李辉, 罗韦, 肖涛, 李兴才, 吴古荣. 兴义鸭MEF2C基因可变剪切体的组织表达差异研究[J]. 农业生物技术学报, 2021, 29(10): 1990-1998.
YE Tao, YANG Hua-Ting, LI Hui, LUO Wei, XIAO Tao, LI Xing-Cai, WU Gu-Rong. Study on Differential Tissue Expression of MEF2C Splice Variants in Xingyi Duck (Anas platyrhynchos). 农业生物技术学报, 2021, 29(10): 1990-1998.
[1] 谌颖莲, 许厚强, 赵佳福, 等. 2018. 影响BLM基因表达的miRNAs筛选及抑制效率研究[J].生物技术, 28(01): 54-59, 6. (Chen L Y, Xu H Q, Zhao J F, et al.2018. Screening and research of inhibit effect on miRNAs regulating BLM gene expression[J]. Biotechnology, 28(01): 54-59, 6.) [2] 程波, 李利, 王林杰, 等. 2012. MEF2基因家族的研究进展[J]. 中国畜牧杂志, 48(15): 70-74. (Cheng B, Li L, Wang L J, et al.2012. Research progress of MEF2 gene family[J]. Chinese Journal of Animal Husbandry, 48(15): 70-74.) [3] 何波. 2006. 猪骨骼肌卫星细胞的培养、鉴定及猪MEF2B和MEF2C基因的初步研究[D]. 硕士学位论文, 华中农业大学, 导师: 熊远著, pp. 36-41. (He B.2006. Culture, identification and biological characteristics of skeletal muscle satellite cells of pig and preliminary study about the porcine MEF2B and MEF2C gene[D]. Thesis for M.S., Huazhong Agricultural University, Supervisor:Xiong Y Z, pp. 36-41.) [4] 黄锎靓. 2012. 肉鸭Myostatin剪切体的表达和调控[D]. 博士学位论文, 四川农业大学, 导师:王继文, pp. 33-36. (Huang K L.2012. Expression and regulation of duck Myostatin gene and its alternative splicesomes[D]. Thesis for Ph.D., Sichuan Agricultural University, Supervisor: Wang J W, pp. 33-36.) [5] 沈并兵. 2015. 山羊MEF2C基因选择性剪接鉴定及其单核苷酸多态性与生长性状的关联分析[D]. 硕士学位论文, 四川农业大学, 导师: 李利, pp. 23-26. (Shen B B.2015. Identification of MEF2C gene alternative splicing and the association of its single nucleotide polymorphisms with growth traits in goat[D]. Thesis for M.S., Sichuan Agricultural University, Supervisor: Li L, pp. 23-26.) [6] 师新彩. 2018. 兴义鸭MEF2C 和MEF2D基因的SNPs分析及原核表达研究[D]. 硕士学位论文, 贵州大学, 导师: 李辉, pp. 22-36. (Shi X C.2018. SNPs analysis and prokaryotic expression of MEF2C and MEF2D genes in Xingyi duck[D]. Thesis for M.S., Guizhou University, Supervisor: Li H, pp. 22-36.) [7] 司建民. 2009. 鸭MEF2家族的克隆及组织表达研究[D]. 硕士学位论文, 四川农业大学, 导师: 王继文, pp. 32-37. (Si J M.2009. Gene clone and tissue expression of duck MEF2 gene family[D]. Thesis for M.S., Sichuan Agricultural University, Supervisor: Wang J W, pp. 32-37.) [8] 王琴. 2017. 山羊MEF2C基因不同转录本的分离鉴定及表达模式研究[D]. 硕士学位论文, 四川农业大学, 导师: 张红平, pp. 7. (Wang Q.2017. Multiple spliced transcripts and differential expression pattern of the myocyte enhancer factor 2C (MEF2C) gene in goat (Capra hircus)[D]. Thesis for M.S., Sichuan Agricultural University, Supervisor: Zhang H P, pp. 7.) [9] 王兴东, 吴晓云, 郭宪, 等. 2020. 牦牛MEF2C基因克隆、生物信息学及表达谱分析[J/OL]. 基因组学与应用生物学, (9): 1-15. (Wang X D, Wu X Y, Guo X, et al. 2020. Cloning bioinformatics analysis and expression profiling of yak MEF2C gene[J/OL]. Genomics and Applied Biology, (9): 1-15.) [10] 展召阳. 2014. 黄牛MEF2C基因遗传变异及其效应分析和组织表达规律研究[D]. 硕士学位论文, 西北农林科技大学, 导师: 陈宏, pp. 28. (Zhan Z Y.2014. Genetic varition and analysis of their effects and tissus expression regular pattern of MEF2C gene in yellow cattle[D]. Thesis for M.S., Northwest A&F University, Supervisor: Chen H, pp. 28.) [11] 赵忠海. 2016. 兴义鸭MEF2A、MEF2B、MEF2C和MEF2D基因克隆与组织时空表达研究[D]. 硕士学位论文, 贵州大学, 导师: 李辉, pp. 28-55. (Zhao Z H.2016. Cloning and spation-temporal expression of MEF2A, MEF2B, MEF2C and MEF2D genes in different tissues of Xingyi duck[D]. Thesis for M.S., Guizhou University, Supervisor: Li H, pp. 28-55.) [12] 朱弘焱, 沈芷伊, 张宇婷, 等. 2018. MEF2C基因调节猪骨骼肌肌纤维的发育表达[J]. 解剖科学进展, 24(04): 350-353. (Zhu H Y, Shen Z Y, Zhang Y T, et al.2018. MEF2C gene regulated the development and expression of skeletal muscle fibers in pig[J]. Progress of Anatomical Sciences, 24(04): 350-353.) [13] Arnold M A, Kim Y, Czubryt M P, et al.2007. MEF2C transcription factor controls chondrocyte hypertrophy and bone development[J]. Developmental Cell, 12(3): 377-389. [14] Black B L, Olson E N.1998. Transcriptional control of muscle development by myocyte enhancer factor-2 (MEF2) proteins[J]. Annual Review of Cell and Developmental Biology, 14: 167-196. [15] Brand N J.1997. Myocyte enhancer factor 2 (MEF2)[J]. International Journal of Biochemistry and Cell Biology, 29(12): 1467-1470. [16] Di Giorgio E, Hancock W W, Claudio B.2018. MEF2 and the tumorigenic process, hic sunt leones[J]. Biochimica Et Biophysica Acta, 1870(2): 261-273. [17] Dietrich J B.2013. The MEF2 family and the brain: From molecules to memory[J]. Cell and Tissue Research, 352(2): 179-190. [18] Leifer D, Li Y L, Wehr K.1997. Myocyte-specific enhancer binding factor 2C expression in human brain development[J]. Journal of Molecular Neuroscience, 8(2): 131-143. [19] Lyons G E, Micales B K, Schwarz J, et al.1995. Expression of MEF2 genes in the mouse central nervous system suggests a role in neuronal maturation[J]. Journal of Neuroscience, 15(8): 5727-5738. [20] Materna S C, Sinha T, Barnes R M, et al.2018. Cardiovascular development and survival require MEF2C function in the myocardial but not the endothelial lineage[J]. Developmental Biology, 445(2): 170-177. [21] Pfaffl M W, Horgan G W, Dempfle L.2002. Relative expression software tool (REST) for group-wise comparison and statistical analysis of relative expression results in real-time PCR[J]. Nucleic Acids Research, 30(9): 1-10. [22] Potthoff M J, Arnold M A, Mcanally J, et al.2007. Regulation of skeletal muscle sarcomere integrity and postnatal muscle function by MEF2C[J]. Molecular and Cellular Biology, 27(23): 8143-8151. [23] Rocha H, Sampaio M, Rocha R, et al.2016. MEF2C haploinsufficiency syndrome: Report of a new MEF2C mutation and review[J]. European journal of medical genetics, 59(9): 478-482. [24] Sun L L, Liu H H, Wang H H, et al.2013. Molecular cloning of the duck MEF2C gene cDNA coding domain sequence and its expression during fetal muscle tissue development[J]. Genes & Genomics, 35(3): 317-325.
