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2025年8月29日 星期五
农业生物技术学报  2020, Vol. 28 Issue (5): 866-875    DOI: 10.3969/j.issn.1674-7968.2020.05.010
  研究论文与报告 本期目录 | 过刊浏览 | 高级检索 |
VEGF在不同繁殖力绵羊卵巢组织中的分布及表达
郭亚军, 王欣荣*
甘肃农业大学 动物科学技术学院,兰州 730070
Distribution and Expression of VEGF in Ovarian Tissues of Sheep (Ovis aries) with Different Fertility
GUO Ya-Jun, WANG Xin-Rong*
College of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, China
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摘要 绵羊(Ovis aries)卵巢中血管生成因子的调控与其血管增生、卵泡发育和黄体形成密切相关,且对绵羊的繁殖性能具有重要的调节作用。研究血管内皮生长因子(vascular endothelial growth factor, VEGF)在小尾寒羊、湖羊及藏羊卵巢中的分布特点与表达规律,探讨VEGF表达与绵羊排卵数、繁殖力的相关性。本研究利用qRT-PCR检测VEGF在不同繁殖力绵羊卵巢中的表达,同时运用免疫组织化学法和蛋白免疫印记(Western blot, WB)结合图像分析软件(image pro plus, IPP)技术,分析VEGF蛋白在卵巢组织中的表达与分布。结果显示,VEGF在绵羊卵巢中均有表达,其高繁殖力绵羊的表达显著高于低繁殖力绵羊(P<0.05)。免疫组织化学结果显示,VEGF阳性信号主要定位在绵羊不同卵泡中的颗粒细胞、颗粒黄体细胞和血管内皮细胞,在卵巢间质和闭锁卵泡颗粒细胞中也呈弱表达;VEGF蛋白阳性信号的强弱与卵泡大小保持一致,且在黄体早期的表达明显高于退化期。研究结果表明,VEGF的高表达能够诱导绵羊卵泡和黄体周围更多的血管新生,提高微血管及毛细血管的通透性,在提高优势卵泡的数量和质量方面发挥了积极作用。本研究为揭示绵羊繁殖力差异的部分遗传机理提供基础资料。
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郭亚军
王欣荣
关键词 不同繁殖力绵羊免疫组化血管内皮生长因子(VEGF)卵巢卵泡发育    
Abstract:The regulation of angiogenic factors in the ovary of sheep (Ovis aries) is closely related to vascular proliferation, follicular development and corpus luteum formation, and has an important regulatory effect on the reproductive performance of sheep. In this study, qRT-PCR was used to detect the expression of VEGF gene in different ovarian ovary, and the expression and distribution of VEGF protein in ovarian tissue was analyzed by Immunohistochemistry and Western blot combined with IPP(image pro plus) technology. The results showed that VEGF was expressed in sheep ovary, and the expression of high fertility sheep was significantly higher than that of low fertility sheep (P<0.05). Studies showed that VEGF-positive signals were mainly localized in granulosa cells, luteal cells and vascular endothelial cells in different follicles of sheep, and were also weakly expressed in ovarian stroma and atresia follicular granulosa cells, the integrated optical density analysis of VEGF found that The expression of VEGF in the ovarian tissues of Small-tailed Han sheep, Hu sheep and Tibetan sheep was consistent. During the development of different follicles, the integrated optical density values of the follicles gradually increased and synchronized with follicular development. The positive signal strength was consistent with the follicle size, and the expression in the early stage of luteal formation was significantly higher than that in the degenerative stage. Researches found that high expression of VEGF could induce more angiogenesis around the follicles and corpus luteum in sheep, which improved the permeability of microvessels and capillaries and played an active role in increasing the number and quality of dominant follicles. This study provides basic information for revealing some of the genetic mechanisms of differences in sheep fecundity.
Key wordsDifferent fertility sheep    Immunohistochemistry    Vascular endothelial growth factor (VEGF)    Ovary    Follicular development
收稿日期: 2019-10-08     
ZTFLH:  S814.1  
基金资助:国家自然科学基金(31560634)
通讯作者: *wangxr@gsau.edu.cn   
引用本文:   
郭亚军, 王欣荣. VEGF在不同繁殖力绵羊卵巢组织中的分布及表达[J]. 农业生物技术学报, 2020, 28(5): 866-875.
GUO Ya-Jun, WANG Xin-Rong. Distribution and Expression of VEGF in Ovarian Tissues of Sheep (Ovis aries) with Different Fertility. 农业生物技术学报, 2020, 28(5): 866-875.
链接本文:  
http://journal05.magtech.org.cn/Jwk_ny/CN/10.3969/j.issn.1674-7968.2020.05.010     或     http://journal05.magtech.org.cn/Jwk_ny/CN/Y2020/V28/I5/866
 
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