Abstract：The zinc finger-homeodomain (ZF-HD) is a family of homologous heterologous box proteins, a plant-specific transcription factor that plays an important role in plant growth and development. In order to explore the function of ZF-HD gene family in the growth and development of Phyllostachys edulis, the family's physicochemical properties, phylogenetic relationship, gene structures and expression patterns were analyzed in detail by bioinformatics based on Phyllostachys edulis genome and related transcriptome data in this study. The results showed that 23 ZF-HD members in the genome with fewer introns (1~2) of P. edulis, and their gene structures and motifs were relatively conservative. The amino acid sequences of ZF-HD gene family had different domains, including zinc-finger (ZF) and homeodomain (HD), which could bind to DNA. Phylogenetic tree showed that ZF-HD gene family could be divided into 6 subfamilies. Combined with the results of multiple sequence alignment and homologous modeling analysis, ZF-HD belonged to the C2H2-type Zn-finger domain super-gene family. The upstream promoter sequences contained multiple cis-acting elements related to hormone response. The collinearity relationship with rice (Oryza sativa) was higher than that with Arabidopsis thaliana, and there was a doubling event of gene duplication. In combination with Gene Ontology (GO) enrichment analysis, RNA-seq data of ZF-HD genes were analyzed under different hormone treatment conditions and in different stages of P. bud development. It was found that ZF-HD could respond to the regulation of exogenous gibberellin (GA) and naphthalene acetic acid (NAA). The high expression of some gene family members in P. edulis shoots indicated that they might be involved in the rapid growth and development of P. edulis shoots. The results of this study will help to reveal the biological functions of the ZF-HD gene family in the rapid growth and development of P. edulis.
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