1 The State Key Laboratory of Subtropical Silviculture, Zhejiang A&F University, Hangzhou 311300, China; 2 Zhejiang Provincial Collaborative Innovation Center for Bamboo Resources and High-efficiency Utilization, Hangzhou 311300, China
Abstract：Retrotransposons, as important parts of Phyllostachys edulis genome, participate in the regulation of the growth and development of Ph. edulis through their own transposonsition, and it is of great significance to obtain active transposons for the molecular breeding of Ph. edulis. In this study,a complete LTR retrotransposons from the Ph. edulis genome that named PHRE9 was characterized. The PCR amplification and bioinformatics analysis of the full length retrotransposon of PHRE9 show that the structural characteristics and evolution patterns as well as the expression patterns under adversity was systematically analyzed. The length of PHRE9 is 6 370 bp, belonging to the Tork branch in Ty1-copia super-family. The transcriptional activity levels of genes in three domains of PHRE9 was measured by qRT-PCR under different treatment conditions, such as DNA methylation inhibitors, irradiation, high salt, high temperature and low temperature. The results showed that PHRE9 expression level was raised under the stress of DNA methylation inhibitors treatment, irradiation, high salt, high temperature (42 ℃), low temperature (4 ℃). The results showed that PHRE9 was a transcription-active transposon. This study could provide basis data for the study of adversity adaptation mechanism and molecular breeding of Ph. edulis.
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