Analysis of Root Physiology and Related Gene Expression in Maize (Zea mays) Under Low Temperature Stress
ZHAO Xun-Chao, GE Sheng-Nan, WEI Yu-Lei, XU Xiao-Xuan, DING Dong, LIU Meng, ZHANG Jin-Jie, SHAO Wen-Jing, XU Jing-Yu*
Heilongjiang Provincial Key Laboratory of Modern Agricultural Cultivation and Crop Germplasm Improvement, College of Agronomy, Heilongjiang Bayi Agricultural University, Daqing 163319, China
Abstract：Low temperature is one of the major abiotic stresses in northern China. In this study, the maize (Zea mays) inbred line 'He 344' was used as the experimental material, which were analyzed changes of antioxidant system, osmotic adjustment substances and related gene expression of root under low temperature at different time points (12 h, 24 h, 3 d and 7 d). The result suggested that compared with the control at low temperature for 7 d, the fresh and dry weights of maize seedling roots were reduced by 47.9% and 66.7%, respectively; Superoxide dismutase (SOD) and peroxidase (POD) activity gradually increased, compared with the control, which increased by 44.8% and 30.1%, respectively; Proline (Pro) and malondialdehyde (MDA) content increased by 77.6% and 83.1%, respectively, compared to the control. A total of 10 ZmSODs genes were screened from transcriptome data of maize seedlings (3 d low temperature treatment vs control). Most ZmSODs genes were up-regulated, among which ZmSOD1, ZmSOD7 and ZmSOD9 were found higher relative expression compared with other genes. A total of 12 up-regulated ZmPODs genes were screened, of which ZmPOD35, ZmPOD92, ZmPOD94 and ZmPOD108 were highly up-regulated. A total of 9 genes encoding lipoxygenase (LOX) enzyme were screened, among which ZmLOX3, ZmLOX5, ZmLOX6 and ZmLOX7 genes were up-regulated. A total of 3 genes related to proline synthesis (delta 1-pyrroline-5-carboxylate synthetase, P5CS) gene were identified, among which ZmP5CS1 and ZmP5CS4 were up-regulated. This study provides a reference for further study on physiological indicators and their related gene expression regulation in the root of maize seedlings under low temperature.
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