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2025年4月3日 星期四
农业生物技术学报  2019, Vol. 27 Issue (8): 1382-1391    DOI: 10.3969/j.issn.1674-7968.2019.08.006
  研究论文与报告 本期目录 | 过刊浏览 | 高级检索 |
从江香猪IGF-1IGF-2基因的克隆、表达及生物信息学分析
朱晓锋1,2, 许厚强1,2,*, 陈伟1,2,3, 谌颖莲1,3, 倪锴1,2, 吴小敏1,2, 倪萌萌1,2, 卢贤君1,2
1 贵州大学 高原山地动物遗传育种与繁殖教育部重点实验室/贵州省动物遗传育种与繁殖重点实验室,贵阳 550025;
2 贵州大学 动物科学学院,贵阳 550025;
3 贵州大学 生命科学学院,贵阳 550025
Cloning, Expression and Bioinformatics Analysis of IGF-1 and IGF-2 Genes in Congjiangxiang Pigs (Sus scrofa)
ZHU Xiao-Feng1,2, XU Hou-Qiang1,2,*, CHEN Wei1,2,3, CHEN Ying-Lian1,3, NI Kai1,2, WU Xiao-Min1,2, NI Meng-Meng1,2, LU Xian-Jun1,2
1 Key Laboratory of Animal Genetics, Breeding and Reproduction in the Platean Mountainous Region Ministry of Education/Key Laboratory of Animal Genetics, Breeding and Reproduction, Guizhou University, Guiyang 550025, China;
2 College of Animal Science, Guizhou University, Guiyang 550025, China;
3 College of Life Science, Guizhou University, Guiyang 550025, China
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摘要 从江香猪(Sus scrofa)具有生长迟缓、个体矮小等特征,与约克夏、杜洛克、长白等猪种相比,在医疗及模式动物的资源挖掘方面有着特殊的价值。本研究通过探讨从江香猪的类胰岛素样生长因子1/2基因(insulin-like growth factors -1/2, IGF-1/2)的生物信息学特征及在从江香猪和大白猪不同组织器官中的表达差异,初步明确从江香猪生长迟缓、个体矮小的原因,为探究微型猪种生长发育分子机制以及人体动物模型的研究提供基础数据。本研究以相同饲养条件下的同龄纯种从江香猪和大白猪作为实验材料,克隆了从江香猪IGF-1 (GenBank No. NM_214256.1)和IGF-2 (GenBank No. NM_213883.2)基因CDS区,并进行结构预测和功能分析;利用qRT-PCR技术分别检测从江香猪和大白猪心脏、肝脏、脾脏、肺脏、肾脏、大肠、小肠、脂肪和背最长肌9个不同组织器官中IGF-1IGF-2基因的mRNA水平。结果发现,从江香猪IGF-1IGF-2基因CDS区分别为435和577 bp,与GenBank登录的野猪(S. scrofa)的基因编码序列均一致;序列分析结果显示,IGF-1和IGF-2蛋白相对分子质量分别为14.390 44和20.312 51 kD,理论等电点分别为9.34和10.00,不稳定系数为65.58和58.64;遗传进化分析得知,与其他12个物种比较,从江香猪IGF-1IGF-2基因与野猪的遗传距离最近;qRT-PCR结果显示,IGF-1基因在从江香猪肺、小肠和大肠中表达量最高(P<0.05),而在大白猪背最长肌中的表达量极显著高于其他组织器官(P<0.01);IGF-2基因在从江香猪肝脏和肺脏中的表达量高,显著高于其他组织器官(P<0.05),而在大白猪中背最长肌和心脏的表达量极显著高于其他组织(P<0.01)。本研究结果揭示了IGF-1IGF-2基因在从江香猪和大白猪中的表达差异性,为进一步探索微型猪种的生长发育机制提供了基础数据。
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朱晓锋
许厚强
陈伟
谌颖莲
倪锴
吴小敏
倪萌萌
卢贤君
关键词 从江香猪类胰岛素样生长因子1/2基因(IGF-1/2)遗传距离基因表达    
Abstract:Congjiang Xiang pig (Sus scrofa) has the characters of short and small body and slow growth rate. For preliminarial exploreation on Congjiang Xiang pig's characters and providing basic data on the molecular mechanism of growth and development of miniature pigs, insulin-like growth factor 1 and 2 (IGF-1 and IGF-2) genes had been analysed by bioinformatics methods as well as their expression level of mRNA in various organs and tiusses from Congjiang Xiang pig and Norther Yorkshinre had been detected. In the current, the coding sequences (CDS) of IGF-1 (GenBank No. NM_214256.1) and IGF-2 (GenBank No. NM_213883.2) genes were had successfully cloned by using Congjiang Xiang pig and Norther Yorkshinre with same feeding condictions and ages and had predicted and dissected on the structures and functions of IGF-1 and IGF-2. The qRT-PCR had been applied to detect mRNA levels of IGF-1 and IGF-2 of Congjiang Xiang pig and North yorkshire in the heart, liver, spleen, lung, kidney, large intestine, small intestine, fat, and longissimus 9 organs or tissues. The results showed that the CDS of IGF-1 and IGF-2 of Congjiang Xiang pig were 435 and 577 bp respectively, which were no difference with CDS sequence of Sus scrofa in GenBank. By sequence prediction, the relative molecular mass, theoretical isoelectric point, and unstable coefficient of IGF-1 and IGF-2 protein resplectively were 14.390 44 and 20.312 5 kD, 9.34 and 10.00, 65.58 and 58.64. Comparing other 12 species by genetic evolution analysis showed that Congjiang Xiang pig was the closest with S. scrofa. The qRT-PCR results displayed that IGF-1 gene had the obviously highest expression level in lung, large intestine, and small intestine of Congjiang Xiang pig (P<0.05) as well as IGF-1 gene had the very significantly highest expression level in longissimus of North Yorkshine (P<0.01) by comparing other organs or tissues. The IGF-2 had obviously higher expression level in liver and lung than other organs and tissues in Congjiang Xiang pig but longissimus and heart of North yorkshine had very significantly higher expression level of IGF-2 gene than other organs or tissues (P<0.01). Our research uncovered the expression difference of IGF-1 and IGF-2 genes between Congjiang Xiang pig and North Yorkshine, which provided basic data for further exploration of the molecular mechanism of growth and development of miniature pigs.
Key wordsCongjiang Xiang pigs    Insulin-like growth factor 1/2 gene (IGF-1/2)    Genetic distance    Gene expression
收稿日期: 2019-03-09     
ZTFLH:  S828.2  
基金资助:国家科技支撑计划(No.2015BAD03B02-3)和黔科合重大专项(黔科合NY字[2013]6008号)
通讯作者: *,gzdxxhq@163.com   
引用本文:   
朱晓锋, 许厚强, 陈伟, 谌颖莲, 倪锴, 吴小敏, 倪萌萌, 卢贤君. 从江香猪IGF-1IGF-2基因的克隆、表达及生物信息学分析[J]. 农业生物技术学报, 2019, 27(8): 1382-1391.
ZHU Xiao-Feng, XU Hou-Qiang, CHEN Wei, CHEN Ying-Lian, NI Kai, WU Xiao-Min, NI Meng-Meng, LU Xian-Jun. Cloning, Expression and Bioinformatics Analysis of IGF-1 and IGF-2 Genes in Congjiangxiang Pigs (Sus scrofa). 农业生物技术学报, 2019, 27(8): 1382-1391.
链接本文:  
http://journal05.magtech.org.cn/Jwk_ny/CN/10.3969/j.issn.1674-7968.2019.08.006     或     http://journal05.magtech.org.cn/Jwk_ny/CN/Y2019/V27/I8/1382
 
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