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2025年4月5日 星期六
农业生物技术学报  2019, Vol. 27 Issue (7): 1246-1258    DOI: 10.3969/j.issn.1674-7968.2019.07.012
  研究论文与报告 本期目录 | 过刊浏览 | 高级检索 |
凹大叶蝉线粒体全基因组序列分析及系统发育关系
俞鹏飞, 李倩, 王梦馨, 潘铖, 崔林, 韩宝瑜*
中国计量大学 浙江省生物计量及检验检疫技术重点实验室,杭州 310018
Analysis of Complete Mitochondrial Genome and Phylogenetic Relationship of Bothrogonia ferruginea
YU Peng-Fei, LI Qian, WANG Meng-Xin, PAN Cheng, CUI Lin, HAN Bao-Yu*
Zhejiang Provincial Key Laboratory of Biometrology and Inspection &Quarantine, China Jiliang University, Hangzhou 310018, China
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摘要 凹大叶蝉(Bothrogonia ferruginea)是我国一类重要的农业经济害虫,测序和分析凹大叶蝉线粒体基因组,并在线粒体水平上探讨凹大叶蝉在头喙亚目中的系统发育地位,对凹大叶蝉系统分类研究具有重要的意义。本研究采用PCR扩增与克隆测序技术,拼接获得凹大叶蝉线粒体基因组全序列,基于蛋白质编码基因的串联序列构建系统发育树。凹大叶蝉线粒体基因组全长15 262 bp (GenBank No. KU167550),包括13个蛋白编码基因(protein-coding genes, PCGs),22个tRNA基因,2个rRNA基因和一段A+T富集区,呈现出与头喙亚目昆虫一致的核苷酸组成和基因排布顺序。基因组碱基A+T含量为76.48%,AT偏度率为0.170 2。基因重叠区域共计14处,基因间隔区域共计10处。A+T富含区内存在ATTTA碱基序列并在其后存在一个典型的微卫星结构(TA)25。叶蝉科昆虫A+T富集区的串联重复区被发现存在ATCTA和CCCTCT两段特殊结构,而角蝉科和其他头喙亚目昆虫未发现此特殊结构。头喙亚目22个种基于线粒体基因组的系统发育关系发现为沫蝉总科+(蜡蝉总科+角蝉总科),凹大叶蝉与檬果褐叶蝉(Idioscopus nitidulus)等4种叶蝉归属于头喙亚目角蝉总科叶蝉科。凹大叶蝉线粒体基因组的基因排布与叶蝉科其他昆虫完全相同,基于线粒体基因组的头喙亚目系统发育关系与传统形态学研究结果一致:凹大叶蝉归属于头喙亚目角蝉总科叶蝉科。本研究为头喙亚目系统分类的深入研究提供了数据资料。
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俞鹏飞
李倩
王梦馨
潘铖
崔林
韩宝瑜
关键词 凹大叶蝉叶蝉科线粒体基因组头喙亚目系统发育    
AbstractBothrogonia ferruginea is an important agricultural and economic pest in China. Sequencing and analyzing the complete mitochondrial genome of B. ferruginea and exploring its phylogenetic status in the suborder Auchenorrhyncha at the mitochondrial level are of great significance to its systematic taxonomy. The complete mitochondrial genome sequence of B. ferruginea was obtained by PCR amplification and sequencing, and its general features and base composition were analyzed. The plyogenetic tree of mitochondrial protein-coding genes of 22 species of Auchenorrhyncha and 2 outgroups was constructed by using the Bayesian inference (BI) and maximum likelihood method (ML). The complete mitochondrial genome of B. ferruginea was 15 262 bp (GenBank No. KU167550), including 13 protein-coding genes (PCGs), 22 tRNA genes, 2 rRNA genes and one control region. The nucleotide composition and gene distribution sequence of B. ferruginea showed the same as insect of Auchenorrhyncha. The A + T content of mitochondrial genome is 76.48%, and the AT-skew is 0.170 2. There were 14 overlapping genes and 10 intergenic regions. All protein-coding genes use the ATN as initiation codon. Majority of PCGs had a complete termination codon (TAA or TAG), except that COⅡ gene terminated with the incomplete stop codon TA. All the predicted tRNAs showed the classic clover-leaf structure, except for the absence of DHU (dihydrouracil) arm of tRNASer(AGN). The A+T rich region contained some conserved sequence (ATTTA) and a typical microsatellite structure (TA)25. Two special structures, ATCTA and CCCTCT, were found in the tandem repeats in the control region of Cicadellidae, but not in the family Membracidae and other Auchenorrhyncha. The phylogenetic relationships of 22 species of Auchenorrhyncha based on mitochondrial genome were identified as Cercopoidea+(Fulgoroidea + Membracoidea). B. ferruginea, Nephotettix cincticeps, Idioscopus nitidulus, Empoasca vitis and Homalodisca vitripennis belonged to Auchenorrhyncha, Membracoidea and Cicadellidae. The gene arrangement pattern of the mitochondrial genome of B. ferruginea was identical to those of other Cicadellidae insects. The phylogenetic relationship of Auchenorrhyncha constructed based on the mitochondrial genome was consistent with those in traditional morphological taxonomy. B. ferruginea belonged to Auchenorrhyncha, Membracoidea and Cicadellidae. Our research provides data for the further study of auchenorrhyncha classification system.
Key wordsBothrogonia ferruginea    Cicadellidae    Mitochondrial genome    Auchenorrhyncha    Phylogeny
收稿日期: 2019-01-31     
ZTFLH:  S433  
基金资助:国家重点研发计划项目(No. 2018YFC1604402)、浙江省自然科学基金(No. LY17C140002)、浙江省基础公益研究计划项目(No. LGN18C160006)和浙江省大学生科技创新项目(No. 017R409055)
通讯作者: hanby15@163.com   
引用本文:   
俞鹏飞, 李倩, 王梦馨, 潘铖, 崔林, 韩宝瑜. 凹大叶蝉线粒体全基因组序列分析及系统发育关系[J]. 农业生物技术学报, 2019, 27(7): 1246-1258.
YU Peng-Fei, LI Qian, WANG Meng-Xin, PAN Cheng, CUI Lin, HAN Bao-Yu. Analysis of Complete Mitochondrial Genome and Phylogenetic Relationship of Bothrogonia ferruginea. 农业生物技术学报, 2019, 27(7): 1246-1258.
链接本文:  
http://journal05.magtech.org.cn/Jwk_ny/CN/10.3969/j.issn.1674-7968.2019.07.012     或     http://journal05.magtech.org.cn/Jwk_ny/CN/Y2019/V27/I7/1246
 
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