Abstract：The complete mitochondrial genome(mtDNA) of mallard was determined by DNA sequencing based on the PCR fragments of 15 pairs of primers.The entire genome was 16 606bp in length.It contained 37 genes(13 protein coding genes,2 rRNA ,22tRNA)and a non-coding control region(D-loop).The characteristics of mtDNA resembled to birds on Anseriforms. Based on the control region in GenBank, Phylogenetic trees constructed using the N-J method indicated that the 13 species of Anatini clustered in three major clades. Among them, the mallards(Anas platyrhychos) ,domestic duck and spot-billed duck(Anas poecilorhyncha) clustered in ones. The results support that domestic ducks origined from mallard and spot-billed duck. Both of mallard and spot-billed duck contributed to domestic duck evolution.
