联系我们 加入收藏
 
年期检索 高级检索
33
2025年4月6日 星期日
  研究论文 本期目录 | 过刊浏览 | 高级检索 |
绵羊mtDNA D-环序列与微卫星DNA遗传多样性和系统发育分析
成述儒,罗玉柱,韩建林,Olivier Hanotte,胡江
甘肃农业大学动物科学技术学院
Analysis of Genetic Diversity and Phylogenetics Using mtDNA D-loop Sequence and Microsatellite DNA Marker in sheep
全文: PDF (762 KB)   HTML (1 KB) 
输出: BibTeX | EndNote (RIS)      
摘要 采用mtDNA D-环序列和微卫星DNA两种标记方法,对9个绵羊群体225只个体进行遗传多样性和系统发育分析。结果表明:两种方法在遗传多样性分析中得出的结论一致,即在研究的9个绵羊群体中青海藏羊的遗传多样性最丰富,而湖羊和岷县黑裘皮羊遗传多样性都较低。但在系统发育分析中,通过mtDNA D-环序列和微卫星DNA座位数据构建系统发育树,结果表现为很大的不同。由于微卫星DNA符合孟德尔遗传规律,能够反映群体间的亲缘关系,构建的系统发育树结构可靠。mtDNA是核外遗传物质,具有母系遗传的特点,在反映群体间的亲缘关系上不具优势。此外,青海细毛羊和甘肃高山细毛羊的育种实践表明它们的遗传来源相似,亲缘关系相近,这与微卫星DNA座位数据构建系统发育树反映的结果一致,因此,在群体的亲缘关系研究上,微卫星DNA数据分析的结果比mtDNA序列分析结果更可信。172个单倍型序列网络关系分析表明研究的9个绵羊群体可能有三个母系起源。
服务
把本文推荐给朋友
加入我的书架
加入引用管理器
E-mail Alert
RSS
作者相关文章
成述儒
罗玉柱
韩建林
OlivierHanotte
胡江
关键词 mtDNA微卫星DNA遗传多样性系统发育绵羊    
Abstract:We analysised the genetic diversity and phylogenesis of 225 individuals of 9 sheep populations using mitochondrial D-loop sequences and microsatellite DNA. The results of the two methods in genetic diversity analysis were consistent. It was that Qinghai Tibetan sheep had rich genetic diversity, but Hu sheep and Minxian Black Fur sheep's genetic diversity were lower than others. While in phylogenesis analysis, phylogenetic trees based on mtDNA D-loop sequence and microsatellite DNA were different. Because microsatellite DNA accords with Mendelian inheritance and can reflect population relationship more reliability. mtDNA is extranuclear inheritance and belongs to maternal inheritance and is not superiority in population relationship analysis. Furthermore, the breeding background of Qinghai Merino and Gansu Alpine Merino showed that they had close relationship. This was accorded with phylogenetic tree constructed by microsatellite DNA. Thus we conclude that microsatellite DNA is more reliable in population relationship analysis. Further network analysis of the phylogenetic relationship of the 172 haplotypes identified from 9 sheep populations supported there are three distinct maternal lineages.
收稿日期: 2008-11-11     
通讯作者: 成述儒   
引用本文:   
成述儒,罗玉柱,韩建林,Olivier Hanotte,胡江. 绵羊mtDNA D-环序列与微卫星DNA遗传多样性和系统发育分析[J]. , 2009, 17(6): 988-994.
链接本文:  
http://journal05.magtech.org.cn/Jwk_ny/CN/     或     http://journal05.magtech.org.cn/Jwk_ny/CN/Y2009/V17/I6/988
 
版权所有 © 2014 《农业生物技术学报》编辑部   京ICP备11035905号-3
地址:北京市海淀区圆明园西路2号中国农业大学生命科学楼1053室 邮编:100193
电话:010-62733684 传真:010-62731615 E-mail: nsjxb@cau.edu.cn