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2025年8月5日 星期二
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河南小麦黑胚病菌链格孢rDNA-ITS序列及系统发育分析
李洪连
河南农业大学植物保护学院
rDNA-ITS sequence and phylogenetic analysis of the pathogens of wheat black point ( Alternaria spp. ) in Henan
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摘要 从河南省具有地域代表性的5个不同地区分离小麦黑胚病原菌Alternaria spp.菌株17株,提取基因组DNA进行rDNA-ITS 5.8S rDNA及其两侧ITS序列扩增,所测菌株的序列同源性为99%-100%。通过与GenBank中登录的链格孢属中的9个种进行系统发育分析,发现所有供分析菌株具有很高的同源性,其中与6个小孢子种A. alternata、A. tenuissima、A. mali、A. gaisen、A. citri、A. arborescens 同源性为98%~100%。但3个大孢子种A .radicina、A. porri和A. solani聚为另外一支,可以明显地与其它小孢子种区分开,且发生变异的个别小孢子种的菌株与大孢子种之间也存在一定的亲缘关系。通过对58个Alternaria 菌株的rDNA ITS1-5.8S-ITS2序列及系统发育分析发现,同一个种内不分地域范围和寄主都有很高的保守性,并且具有各自的特异位点,所以该段序列可以作为一些种的分类鉴定、分子标记及系统发育的重要依据。比对分析的结果也进一步从分子角度验证引起河南省小麦黑胚病的病原菌是链格孢属的相似的小孢子种。
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李洪连
关键词 小麦黑胚病,链格孢,rDNA-ITS,同源性,系统发育分析    
Abstract:17 samples of Alternaria spp. which is the pathogen of wheat black point were collected from five different places in Henan, and their genomic DNAs were extracted by CTAB. The ribosomal DNA internal transcribed spacer (ITS) region of the genomic DNAs was amplified and sequenced, the homology of the 17 isolates was 99%-100%. The sequences were analyzed by phylogenesis with 9 species of Alternaria spp. from GenBank. The result showed that all the isolates had high homology The homology of the 6 small-spored species A. alternata、A. tenuissima、A. mali、A. gaisen、A. citri、A. arborescens is 98%-100%, and the other three large-spored species A. radicina、A. porri and A. solani were separated clearly. There are also some certain relative relationship between the mutation isolates of small-spored species and the large-spored ones. The rDNA ITS1-5.8S-ITS2 of all the isolates did not have the regionally and host characters but had high conservation with special sequences respectively, which can be the important evidence in the classification, identification, molecular marker and phylogenetic analyses of some certain species of epiphyte. The result also prove by molecular evidence that some similar small-spored species of Alternaria spp. are the pathogens of wheat black point in Henan.
Key wordswheat black point; Alternaria spp.; ITS1-5.8S-ITS2; homology; phylogenetic analysis
收稿日期: 2009-04-22     
通讯作者: 李洪连   
引用本文:   
李洪连. 河南小麦黑胚病菌链格孢rDNA-ITS序列及系统发育分析[J]. , 2010, 18(3): 445-455.
链接本文:  
http://journal05.magtech.org.cn/Jwk_ny/CN/     或     http://journal05.magtech.org.cn/Jwk_ny/CN/Y2010/V18/I3/445
 
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