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2025年8月29日 星期五
  2018, Vol. 26 Issue (11): 1872-1879    
  研究论文与报告 本期目录 | 过刊浏览 | 高级检索 |
生长素介导细胞分裂素(玉米素)调控杜鹃兰侧芽萌发
吕享,叶睿华,田海露,吉宁,刘思佳,张明生
贵州大学
Auxin regulated lateral buds germinationin Cremastra appendiculata via the regulation of cytokinin (Zeatin)
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摘要 杜鹃兰(Cremastra appendiculata)为多年生珍稀药用植物,自然条件下有性繁殖困难,营养繁殖因新生假鳞茎顶端抑制而不能有效进行“分枝”发育扩增假鳞茎,致使其繁殖系数极低。为探讨杜鹃兰假鳞茎成串生长的内在机制,本研究通过打顶及生长素运输抑制剂N-1-氨甲酰苯甲酸萘酯(N-1-naphthylphthalamic acid, NPA)和2,3,5-三碘苯甲酸(2,3,5-triiodibenzoic acid, TIBA)处理杜鹃兰假鳞茎,借此改变其内源植物激素水平以促进假鳞茎侧芽生长。研究结果表明,打顶及生长素运输抑制剂处理6 d后,侧芽内生长素水平显著降低(P<0.05),细胞分裂素水平则显著提高(P<0.05),且细胞分裂素水平的升高与显著上调细胞分裂素合成酶的关键基因—异戊烯基转移酶基因(isopentenyl transferase, IPT)的表达是一致的。处理40 d后,打顶、NPA和TIBA使二年生假鳞茎侧芽萌发率从0%分别提高到95%、85%和90%,以及三年生假鳞茎也分别提高到15%、76%和75%;处理90 d后,单株出苗数分别可达到对照的2.20、3.13和3.05倍。然而,外施玉米素(zeatin, ZT),仅使二年生和三年生假鳞茎萌发率提高至51%和35%,并未提高出苗率。可见,生长素可能通过调控IPT的表达,以此调控细胞分裂素水平,从而间接控制杜鹃兰侧芽的生长。本研究结果有助于提高杜鹃兰的繁殖系数,为地下茎顶端优势的理论研究提供了参考。
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吕享
叶睿华
田海露
吉宁
刘思佳
张明生
关键词 杜鹃兰生长素细胞分裂素侧芽生长素运输抑制剂    
Abstract:Cremastra appendiculata is a perennial rare medicinal plant. Under natural conditions, it's hard to reproduce sexually, and the vegetative reproduction cannot effectively branch development to increase the number of pseudobulb due to apical inhibition of newborn pseudobulb. These factors lead to a very low breeding coefficient. Therefore, to explore internal mechanism of pseudobulbs growth in cluster, the pseudobulbs of C. appendiculata were treated by decapitation, auxin transport inhibitors N-1-naphthylphthalamic acid (NPA) and 2, 3, 5-triiodibenzoic acid (TIBA). In this way, endogenous plant hormone levels might be changed to promote the growth of lateral buds of C. appendiculata. After decapitation and application of auxin transport inhibitors (NPA and TIBA) treated the pseudobulb of C. appendiculata for 6 days, the results showed that auxin levels in the lateral buds were significantly reduced (P<0.05), and cytokinin levels were obviously increased (P<0.05). The results of qRT-PCR showed that cytokinin levels increased because of rapidly up-regulated key genes of cytokinin synthetase (isopentenyl transferase, IPT) after treatments. After the decapitation, NPA and TIBA treating for 40 d, the germination rate of the biennial pseudobulbs in pseudobulbs string were increased from 0% to 95%, 85% and 90%, respectively, and the triennial pseudobulbs were increased from 0% to 15%, 76% and 75%, respectively. For the treatments of decapitation, NPA and TIBA, the number of seedlings per plant could reach to 2.20, 3.13 and 3.05 times of the control after 90 d, respectively. The application of zeatin promoted the germination of lateral buds of C. appendiculata in a certain extent (biennial: 51%; triennial: 35%), but did not increase the rate of seedling emergence, which indicated that cytokinin promoted the germination of lateral buds, but could not promote to continued growth. The change of auxin levels was necessary for the continued growth of lateral buds. The results of this study indicated that auxin might regulate transcriptional level of IPT gene to control cytokinin level, and indirectly regulated lateral buds development and growth of C. appendiculata. This research increases the propagation coefficient of C. appendiculata, and provides a reference for the apical dominance theory of underground stems.
Key wordsCremastra appendiculata    Auxin    Chlorophyll content    Lateral buds    Auxin transport inhibitors
收稿日期: 2018-03-23      出版日期: 2018-10-24
基金资助:;国家重点研发计划课题;贵州省科技计划重大专项课题;国家喀斯特石漠化防治工程技术研究中心建设;贵州省科技创新人才团队建设专项资金;贵州省教育厅创新群体重大研究项目;贵州省研究生科研基金
通讯作者: 张明生     E-mail: mszhang@gzu.edu.cn
引用本文:   
吕享 叶睿华 田海露 吉宁 刘思佳 张明生. 生长素介导细胞分裂素(玉米素)调控杜鹃兰侧芽萌发[J]. , 2018, 26(11): 1872-1879.
链接本文:  
http://journal05.magtech.org.cn/Jwk_ny/CN/     或     http://journal05.magtech.org.cn/Jwk_ny/CN/Y2018/V26/I11/1872
 
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