Abstract:Abstract: This study was aimed to compare the contents of endogenous cytokine (ZRs、DHZRs、iPAs ) and auxin (IAA) in decussate and alternative maize by the ELISA method .Two sets of near isogenic lines of 8701d, 8701D and H4d, H4D were used in these examinations. In this study, the results showed that the maize with different phyllotary leaf had same level of endogenous cytokine (ZRs、DHZRs、iPAs ) and auxin in mature pollen ,different development leaf and young embryo. However,the contents of above cytokine in immature male spikes from decussate plants and SAMs were great higher than that in isogenic alternative maize. At the same time, there was no significant difference in the level of IAA among them. Further analysis indicated that the value of CTK/IAA in decussate maize was not only dominant higher than in alternative maize, but also was increased accompanied with phyllotary changes from alternative to decussate. Taken together, we can draw a conclusion that there is close relativity between the decussate phenotype and the level of SAM cytokines. The stabilization and change of CTK/IAA is an important factor which regulates phyllotary development.The producing of decussate mutant is relative to the change of endogenous hormone content in growthing tissue.