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2025年8月20日 星期三
  2016, Vol. 24 Issue (2): 215-223    
  研究论文与报告 本期目录 | 过刊浏览 | 高级检索 |
黄瓜生长素受体同源基因CsAFB和CsTIR在拟南芥中的转化与功能表达
姚丽琴,陈哲皓,鲍林文,何露霞,王利琳
杭州师范大学
Transformation and Functional Expression of Cucumber (Cucumis sativus) Auxin Receptor Homologous Genes CsAFB/CsTIR in Arabidopsis thaliana
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摘要 植物生长素受体是生长素信号通路中的重要因子。基于前期克隆得到了黄瓜(Cucumis sativus)生长素受体同源基因生长素信号F-box蛋白基因(auxin signaling F box protein, CsAFB)和转运抑制响应基因(transport inhibitor response, CsTIR),为进一步证实和研究这2个基因的功能,本研究利用拟南芥(Arabidopsis thaliana)Col-0野生型和生长素受体编码基因功能缺陷突变体tir1-1为材料,导入黄瓜生长素受体同源基因,获取纯合转基因系。检测发现,拟南芥突变体tir1-1转入CsAFB/TIR基因后根系发育、外源生长素敏感性和细胞伸长反应均恢复至野生型水平。检测发现,野生型拟南芥中过量表达黄瓜CsAFB/TIR,尤其是CsTIR基因,植株主根伸长明显受抑,侧根数量剧增,子叶下卷,叶柄上翘,真叶叶缘向离轴面弯曲,顶端优势明显。本研究表明,CsAFB/TIR基因功能类似拟南芥TIR1基因,为黄瓜生长素受体同源基因;过量表达该类基因通过增加生长素受体数量、扩大生长素信号的方式参与调控植物生长发育;为进一步在黄瓜中研究生长素受体功能及作用机理提供理论依据。
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姚丽琴
陈哲皓
鲍林文
何露霞
王利琳
关键词 生长素受体CsAFBCsTIR表型恢复基因功能    
Abstract:Plant auxin receptor is the key point in auxin signaling pathways. We had cloned the auxin receptor homologous genes in cucumber (Cucumis sativus) in the previous work with the name of auxin signaling F box protein (CsAFB) and transport inhibitor response(CsTIR). In order to verify and further explore their function, we transformed them into both TIR1 loss-of-function mutant tir1-1 and wild-type Col-0 Arabidopsis thaliana and obtained homozygous transgenic plants, respectively. With the transformation of CsAFB and CsTIR into tir1-1 mutant, the abnormal phenotypes of root system development, exogenous auxin sensitivity and cell elongation response in tir1-1 could be rescued. Overexpression of CsAFB/CsTIR in wild-type A. thaliana, especially CsTIR, led to inhibited elongation of primary root, increased numbers of lateral roots, curled cotyledons, upward petioles, bending leaf margins towards abaxial surface and had obvious apical dominance. This work illustrated that CsAFB/TIR functioned similarly as AtTIR1 in Arabidopsis. These genes were probably auxin receptor homologous genes in cucumber. Overexpression of CsAFB/TIR could enhance the number of auxin receptors, amplify auxin signal and regulate plant growth and development in A. thaliana. This work will contribute to the further study of CsAFB/TIR functions and regulation mechanisms in cucumber.
Key wordsAuxin receptor    CsAFB    CsTIR    Phenotype rescue    Gene function
收稿日期: 2015-04-15      出版日期: 2015-12-29
基金资助:浙江省自然科学基金; 杭州师范大学启动经费
通讯作者: 王利琳     E-mail: wang_208@163.com
引用本文:   
姚丽琴 陈哲皓 鲍林文 何露霞 王利琳. 黄瓜生长素受体同源基因CsAFB和CsTIR在拟南芥中的转化与功能表达[J]. , 2016, 24(2): 215-223.
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http://journal05.magtech.org.cn/Jwk_ny/CN/     或     http://journal05.magtech.org.cn/Jwk_ny/CN/Y2016/V24/I2/215
 
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