Abstract：Abstract Osmotin (OSM) is a multifunction protein present in wide plant species. It is inferred that OSM regulates plant adaption to stress through involving in plant growth and development, However, direct evidence linking between OSM and growth is rare. Based on StOSM-3b cDNA of potato (Solanum tuberosum) isolated in previous study, an antisense StOSM-3b vector, aiming to inhibit OSM expression, was constructed in this study. The antisense StOSM-3b was transformed into potato by Agrobacterium-mediated approach. A positive transgenic line was screened by β- glucuronidase (GUS) staining. Analysis on data assayed showed that accumulations of StOSM-3b mRNA and OSM contents by enzyme linked immunosorbent assay (ELISA) in the transgenic potato leafs was 25% and 52% of that in control, respectively. This indicated that the antisense StOSM-3b partially inhibited expression of StOSM-3b and down-regulated OSM accumulation in the transgenic potato. OSM down-expression led to a tardy growth, dwarfing and no tuber-formation phenotype with disorder in growth rhythm. The results provide a direct evidence to indicate that osmotin involves in plant growth and development.
[1] Castillo RA, Herrera C, Ghislain M, et al. 2005. Organization of phenylalanine ammonia lyase (PAL), acidic PR-5 and osmotin-like (OSM) defence-response gene families in the potato genome. Mol Genet Genomics， 274: 168-179. [1] [2] Kumar SA, Kumari HG. Kishor K, et al. 2015. Osmotin: a plant sentinel and a possible agonist of mammalian adiponectin [J]. Front Plant Sci. 6: 163-168. doi: 10.3389/fpls.2015.00163. [3] Kumar V, Mills DJ, Anderson JD, et al. 2004. An alternative agriculture system is defined by a distinct expression profile of select gene transcripts and proteins[J]. Proc Natl Acad Sci U S A. 20;101(29):10535-40. 6. [4] Kononowicz AK, Nelson DE, Singh NK, et al. 1992. Regulation of the Osmotin Gene Promoter[J]. Plant Cell. 4(5):513-524. [5] Liu JF, Feng XJ, Cui XQ, et al. Expression of E. coli glgB in Potatoes Generating Tuber Starches with Various Features. Molecular Plant Breeding. 2012 , 3:6,57-62. [6] Mukherjee AK, Carp MJ, Zuchman R, et al. 2010. Proteomics of the response of Arabidopsis thaliana to infection with Alternaria brassicicola[J]. J Proteomics. 10;73(4):709-20. doi: 10.1016/j.jprot.2009.10.005. [7] Qiao LB，Fan HH，Zhang XP，et al. 2015. Eleven StOSMs Genes in the Potato Genome Response to Water Deficiency[J].Bioscience Methods. 6（1）：1-13. doi: 10.5367/bm.2051.06.0001. [8] Thomas JC, Smigocki AC, Bohnert HJ. 1995. Light-induced expression of ipt from Agrobacterium tumefaciens results in cytokinin accumulation and osmotic stress symptoms in transgenic tobacco[J]. Plant Mol Biol 28(5):965-969. [9] Tachi H, Fukuda-Yamada K, Kojima T, et al. 2009. Molecular characterization of a novel soybean gene encoding a neutral PR-5 protein induced by high-salt stress[J]. Plant Physiol. Biochem. 47, 73–79. [10] Tada Y, Kashimura T. 2009.Proteomic analysis of salt-responsive proteins in the mangrove plant, Bruguiera gymnorhiza[J]. Plant Cell Physiol. 50(3):439-46. doi: 10.1093/pcp/pcp002. [11] Weber RLM, Wiebke-Strohm B, Bredemeier C, et al. 2014. Expression of an osmotin-like protein from Solanum nigrum confers drought tolerance in transgenic soybean[J]. BMC Plant Biol. 14(1):1-9. [12] Wiesel L, Davis JL, Milne L, et al. 2015. A transcriptional reference map of defence hormone responses in potato[J]. Sci Rep. 19;5:15229. doi: 10.1038/srep15229. [13] Zhu JK. 2016. Abiotic Stress Signaling and Responses in Plants[J]. Cell. 6;167(2):313-324. doi: 10.1016/j.cell.2016.08.029.
