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2025年8月18日 星期一
  2016, Vol. 24 Issue (1): 61-67    
  研究论文与报告 本期目录 | 过刊浏览 | 高级检索 |
绵羊TSHR基因第10外显子T481C位点多态性分析
高磊,沈敏,赵赓,甘尚权,杨井泉
新疆农垦科学院
Polymorphism Analysis of T481C Locus of TSHR Gene Exon 10 in Sheep (Ovis aries)
1, 1, 1, 1
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摘要 促甲状腺激素受体(thyroid-stimulating hormone receptor, TSHR) 在光周期介导的哺乳动物季节性繁殖过程中起着非常重要的作用,但在绵羊(Ovis aries)中的遗传特性与分子机制仍不明晰。本研究以筛选的绵羊TSHR基因第10外显子T481C位点SNP为候选分子标记,利用多聚酶链式反应-单链构象多态(polymerase chain reaction-single strand conformation polymerase, PCR-SSCP)技术,检测该位点在繁殖特性不同的阿勒泰羊、中国美利奴羊、萨福克羊、多浪羊以及湖羊和小尾寒羊群体中的多态性。结果表明,绵羊TSHR基因在第10外显子481 bp处发生了T-C突变,为同义突变,SSCP检测出3种基因型:TT、TC和CC,T481C位点的TT基因型在常年发情的湖羊、小尾寒羊和多浪羊群体中属于优势基因型,而在季节性繁殖的阿勒泰羊、萨福克羊和中国美利奴羊群体中比率最低,仅为8%;方差分析结果表明,常年发情的多浪羊、小尾寒羊和湖羊群体T481C位点的基因型频率与季节性繁殖的阿勒泰羊、萨福克羊和中国美利奴羊存在极显著差异(P<0.01)。研究结果提示,绵羊TSHR基因第10外显子T481C位点多态性在常年发情与季节性繁殖绵羊群体中分布存在较大差异,该SNP可作为一个理想的分子标记应用于常年发情绵羊品种选育。
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高磊
沈敏
赵赓
甘尚权
杨井泉
关键词 绵羊TSHR基因季节性繁殖多态    
Abstract:Thyroid-stimulating hormone receptor (TSHR) may play important roles in process of seasonal breeding in mammals mediated by photoperiod, but the hereditary character and molecular mechanism in sheep (Ovis aries) are still unclear. Therefore, the T481C locus on exon 10 of TSHR in our unpublished chip date was chosen as candidate SNP, polymerase chain reaction-single strand conformation polymerase (PCR-SSCP) method was used to detect genotypes in six sheep breeds which have differences in reproductive characteristics (Altay, Chinese Merino, Suffolk, Duo Lang, Hu and Small Tail Han Sheep). The results showed that the T-C point mutation was detected in 481 bp of exon 10 of TSHR gene in sheep, which was a silent mutation, three genotypes (TT, TC and CC) were found, TT genotype at T481C locus was preponderant genotype in non-seasonal breeding sheep flocks (Small tail han sheep and Duo lang sheep), while the lowest percentage of the genotype in seasonal breeding sheep flocks(Altay, Chinese merino and Suffolk) is only 8%. Analysis of variance suggested T481C was significantly different between the allele frequencies in seasonal and non-seasonal breeding sheep breeds(P<0.01). These results suggest that there is a big difference in the SNP distribution between seasonal breeding and non seasonal breeding sheep populations, and the SNP can be used as an ideal molecular marker in non-seasonal reproduction sheep breeding.
Key wordsSheep(Ovis aries)    TSHR gene    Seasonal breeding    Polymorphism
收稿日期: 2015-05-11      出版日期: 2015-11-23
基金资助:新疆地方绵羊品种季节性繁殖的分子机理研究;基于卵巢组织差异表达基因的多浪羊常年发情的分子遗传基础;绵羊重要经济性状关键基因的挖掘及其功能验证
通讯作者: 沈敏     E-mail: shenmin0993@sina.com
引用本文:   
高磊 沈敏 赵赓 甘尚权 杨井泉. 绵羊TSHR基因第10外显子T481C位点多态性分析[J]. , 2016, 24(1): 61-67.
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http://journal05.magtech.org.cn/Jwk_ny/CN/     或     http://journal05.magtech.org.cn/Jwk_ny/CN/Y2016/V24/I1/61
 
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