Abstract:MYB gene family are important transcription factors involving in plant responses to biotic and abiotic stresses by controlling the expression of diverse functional genes. Utilizing genomic sequences of diploid Gossypium raimondii, a new cotton MYB transcription factor gene GhMYB11 (GenBank accession No. HQ234875.1) was cloned from upland cotton(G. hirsutum) cultivar Lumianyan 32. The full length of GhMYB11 cDNA was 1 001 bp coding an open reading frame (ORF) of 828 bp. There were 11 SNPs (single nucleotide polymorphism) between the ORF sequences of GhMYB11 and G. raimondii 001G177100.1, of which 7 SNPs caused amino acid alternation. Phylogenetic analysis showed that GhMYB11 was highly similar (E value=7e-83 and 1e-90, respectively) to AtMYB13/14, which were 2 important factors involving in ABA synthesis and signal transduction in Arabidopsis thaliana, with 2 highly conservative MYB DNA binding domains R2R3 and a transactivation domain. Real-time quantitative PCR analysis revealed that after Verticillium dahliae strain VD8 inoculated, GhMYB11 expression of cotton seedlings very significantly up-regulated by 14.49 times(P<0.01) in cotton leaves and by 5~6 times significantly(P<0.05) after 10% PEG6000, 150 mmol/L NaCl and 200 mmol/L H2O2 treated, respectively. The result suggested that GhMYB11 maight play a key role in cotton responding to biotic and abiotic stresses and could be treated as an important candidate gene in genetic improvement for stress resistance of upland cotton cultivars. This work provides basic materials for cotton stress tolerance enhancement through genetic engineering.
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