Cloning of Ribosomal Protein S15a Gene (TaRPS15a) and Its Expression Patterns Based on Temporal-spatial in Multi-ovary Line of Wheat (Triticum aestivum)
Abstract：To study the effect of 40S ribosomal protein S15a (RPS15a) on the formation of multi-ovary in wheat, the EST highly similar to RPS15a gene from the multi-ovary SSH-cDNA library was used as a query probe to blastn the GenBank databases. Based on the assembled homologous cDNA sequence, both cDNA and DNA sequences encoding a RPS15a were isolated and characterized from multi-ovary line by RT-PCR. Furthermore, expression patterns of the gene in different material, different development stages of ears in multi-ovary line and different tissues of multi-ovary line were analyzed by semi-quantitative RT-PCR. Results showed that the cDNA sequence (GenBank accession No.HM055513) was 413 bp in length and the open reading frame encoded a peptide of 131 amino acids. The DNA sequence (GenBank accession No.HM063421) was 642 bp in length, which contained three extrons and two introns. According to expression analysis, the expression of the gene in 2~4 mm ears of multi-ovary line was higher than that in mono-ovary line. This gene was upregulated with the development of young ear, and the highest levels of expression at period of 8~9 mm. Besides, the gene was expressed in various tissues and relatively higher at the stem shoot meristem than that of other parts examined. Thus conclusively the RPS15a gene is differentially expressed in multi-ovary and mono-ovary lines and may involve in the information of multi-ovary.
