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2025年8月8日 星期五
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JAK2/STAT3信号通路对小鼠骨骼肌发育和能量代谢相关基因mRNA表达的影响
马佩云1,孙超2,张忠品3,张朝3,齐仁立 3
1. 西北农林科技大学动物科技学院
2. 西北农林科技大学
3.
Effects of JAK2/STAT3 Signal Pathway on mRNA Expression of Skeletal Muscle Development and Energy Metabolic Related Genes in Mouse
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摘要 利用AG490特异性抑制剂阻断Janus蛋白酪氨酸激酶2(JAK2)/信号转导和转录激活子3(STAT3)信号通路,研究该通路对骨骼肌发育和能量代谢相关基因mRNA表达水平的影响。雄性健康昆明小白鼠(Mus musculus),用JAK2特异性抑制剂AG490 连续腹腔注射15 d,定期测体重和体温,小鼠处死后取腿肌提取总RNA,利用RT-PCR检测JAK2/STAT3信号通路关键因子JAK2和STAT3、骨骼肌发育相关基因成肌分化因子(MyoD)和生肌决定因子(Myf5)及能量代谢相关基因肝X受体(LXRα)和解偶联蛋白3(UCP3)mRNA表达。结果显示,与对照组相比,处理组小鼠体重下降,差异显著(P<0.05);小鼠体温维持在正常浮动范围内;JAK2和 STAT3 mRNA表达量下降,差异显著(P<0.05);骨骼肌发育相关基因MyoD 和Myf5 mRNA表达量下降,差异极显著(P<0.01);能量代谢相关基因LXRα和UCP3 mRNA表达量下降,差异极显著(P<0.01)。 结果提示JAK2/STAT3信号通路可通过降低骨骼肌发育和能量代谢相关基因的mRNA表达而调节骨骼肌发育和能量代谢。
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马佩云
孙超
张忠品
张朝
齐仁立
关键词 小鼠JAK2/STAT3通路骨骼肌发育能量代谢    
Abstract:The mRNA expression of muscle development and energy metabolic related genes were investigated by inhibiting janus protein-tyrosine kinase 2(JAK2)/signal transducer and activator of transcription 3(STAT3) signal pathway using AG490. The tentative male Kunming mice (Mus musculus) were treated as intraperitoneal injection of AG490 for two weeks. After execution, the leg muscled was surgically removed and total RNA was extracted. Real-time quantitative PCR was used to examine the mRNA expression of the key factors in JAK2/STAT3 signal pathway JAK2 and STAT3, the growth relative genes of skeletal muscle myogenic determination(MyoD)and myogenic factor 5 (Myf5), and energy metabolic relative genes Liver X receptors(LXRα)and Uncoupling proteins (UCP3). Compared with control, the body weight of mice were significantly decreased (P<0.05); the body temperature was stable; the expression of JAK2 and STAT3 mRNA were significantly decreased(P<0.05); the expression level of LXRα, UCP3, MyoD5 and Myf5 mRNA were decreased extremely significant(P <0.01). JAK2/STAT3 signal pathway may regulate the growth of skeletal muscle and energy metabolism by decreasing the mRNA expression levels of the growth of skeletal muscle development and energy metabolic relative genes.
Key wordsmouse    JAK2/STAT3 signal pathway    skeletal muscle development    Energy metabolism
收稿日期: 2009-05-25     
通讯作者: 孙超   
引用本文:   
马佩云1,孙超2,张忠品3,张朝3,齐仁立 3. JAK2/STAT3信号通路对小鼠骨骼肌发育和能量代谢相关基因mRNA表达的影响 [J]. , 2010, 18(5): 951-955.
链接本文:  
http://journal05.magtech.org.cn/Jwk_ny/CN/     或     http://journal05.magtech.org.cn/Jwk_ny/CN/Y2010/V18/I5/951
 
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