Abstract：Glucagon-like peptide-2(GLP-2) can promote the growth of intestine, enhance nutrient absorption and repair intestinal injures. In this study, porcine glucagon-like peptide-2 (pGLP-2) microspheres were prepaired by Water in oil in water(W/O/W)method and solid in oil in water(S/O/W)method. A better one was chosen to investigate the potential therapeutic effects on intestinal injures of colitis in mice(Mus musculus) induced by dextran sulfate sodium (DSS). The results showed that microspheres prepared by W/O/W method could encapsulate 60% pGLP-2 with (54.19±17.28) μm in diameter while the drug burst was 65.1% and the surface was porous; Microspheres prepared by S/O/W method could encapsulate 72% pGLP-2 with (31.17±8.17) μm in diameter and 15.58% in drug burst. BALB/c mice were inoculated with 3% DSS solution for 7 d to induce colitis successfully. DSS inoculated mice were treated with pGLP-2 microspheres (10 mg per mice) prepared by S/O/W method in the third day. DSS+pGLP-2 microspheres group significantly decreased body weight loss and colonic damage and increased colon length (P＜0.05). Villus height and villus height /crypt depth ratio in mice of DSS+microspheres group significantly increased compared with that of DSS-only inoculated group and water group (P＜0.05). This study demonstrates pGLP-2 microspheres prepared by S/O/W method had higher encapsulation efficiency, lower drug burst and more stable than that of microspheres prepared by W/O/W method. Administration pGLP-2 microspheres once reduced the severity of colonic injury in murine colitis. The potencies of this product highlight its potential as therapeutic agent for intestinal diseases in piglet.
