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2025年8月29日 星期五
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抗粘液病毒基因(Mx )S631N多态位点与白耳鸡免疫性状和生产性能的关联分析﹡
韩威
中国农业科学院家禽研究所
Association analysis between myxo-virus resistance gene (Mx) S631N polymorphism site and immune traits and production performance of BAI chicken breeds
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摘要 本试验通过PCR-RFLP方法检测抗粘液病毒基因(Mx)S631N多态位点的等位基因A和G在白耳鸡群体中的分布,通过选配组建三种不同基因型群体(AA,AG,GG),免疫后分别于35、42、49、70、100日龄采用血凝和血凝抑制方法测定不同基因型个体的禽流感(AI)和新城疫(ND)抗体滴度,并测定不同基因型个体的90日龄体重、开产日龄和72周龄产蛋数;分析该多态位点与免疫性状和生长性状的关联。结果表明,在白耳鸡群体中Mx基因S631N多态位点的等位基因频率处于Hardy-Weinberg平衡状态(P>0.05),3种不同基因型频率分别为0.233(AA),0.517(AG)和0.250(GG);在测定的日龄内不同基因型个体的AI抗体滴度和ND抗体滴度水平呈现出类似的增长和消减变化规律,在多个测定日龄内AA基因型个体的AI抗体滴度和ND抗体滴度水平显著高于(P<0.05)AG和GG基因型个体;关联分析结果表明该多态位点与49日龄时的ND抗体滴度峰值和70日龄时的AI抗体滴度峰值间均存在显著关联(P<0.05),但不同基因型个体间的90日龄平均体重、开产日龄和72周龄产蛋数差异均不显著(P>0.05)。研究结果证实Mx基因S631N多态位点可以作为提高抗病力的重要候选标记位点。
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韩威
关键词 白耳鸡抗粘液病毒基因免疫性状生产性状关联分析    
Abstract:In the experiment, PCR-RFLP technique was used to detect the alleles distribution of S631N polymorphism site of myxo-virus resistance gene (Mx) in BAI chicken populations. Populations with different genotypes were constructed by selected mating method. After vaccine were injected the Newcastle Disease (ND) and Avian Influenza (AI) antibody titers of individuals with different genotypes were respectively measured at 35, 42, 49, 70 and 100 days of age using haemagglutination activity (HA) text and hemagglutination inhibition (HI) text. The body weight of 90 days, age at first laying and egg number at 72 weeks of individuals with different genotypes were also measured. The association between the polymoephism site and immune traits and production performance were analyzed. The resulted showed the allele frequencies at this locus was in Hardy-Weinberg equilibrium (P>0.05) in BAI populations, the frequencies of three different genotypes were 0.233(AA), 0.517(AG) and 0.250(GG). In tested days, the ND and AI antibody titers with different genotypes had the same increased and decreased laws, but individuals with AA genotypes had higher levels (P<0.05) than that of AG and GG genotypes in several tested days. The association analysis showed this polymorphism site had significant effect (P<0.05) on peak ND antibody titer of 49 days and AI antibody titer of 70 days. However, the body weight of 90 days, age at first laying and egg number at 72 weeks had no variation among individuals with different genotypes. The results identified the S631N polymorphism site of Mx gene as an important candidate mark for improving antiviral activity.
Key wordsBAI chicken    mx gene    immune traits    production performance    association analysis
收稿日期: 2009-05-25     
通讯作者: 韩威   
引用本文:   
韩威. 抗粘液病毒基因(Mx )S631N多态位点与白耳鸡免疫性状和生产性能的关联分析﹡[J]. , 2010, 18(3): 556-561.
链接本文:  
http://journal05.magtech.org.cn/Jwk_ny/CN/     或     http://journal05.magtech.org.cn/Jwk_ny/CN/Y2010/V18/I3/556
 
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