Abstract：Proteome difference between high royal jelly producing bees (A. mellifera L.) and Chinese honeybees (A. cerana cerana F.) was investigated by two-dimensional gel electrophoresis, and some proteins were deduced by comparing with the already identified royal jelly proteome . Significant higher protein spots (93) in royal jelly of high royal jelly producing bee were found than those of in Chineses honeybee’s (70). Their molecular weight ranged from 50 to 80 kDa, and pI 5.0-8.0. Thirty protein spots were resolved to two images, while others were unique to each sample. Comparing with the already identified royal jelly proteome, it can be deduced that these well resovled proteins were likely MRJP1 (major royal jelly protein 1), MRJP2, MRJP3 and glucose oxidase. Protein abundance analysis of proteins present on two samples showed that 4 proteins of Chinese honeybee significantly higher than those of high royal jelly producing bees, but 7 spots showed a reverse relation. The results suggest that there are significant differences between royal jelly from high royal jelly producing bees and Chinese honeybees in term of proteins composition and abundance.
