Abstract：In this paper, we investigated the secondary somatic embryo induction from primary somatic embryo which developed at globular stage to cotyledonary stage. The highest secondary embryo induction rate of 85.61％ was obtained from the treatment with 1µM 6-BA. High content of the sucrose induced repetitive somatic embryogenesis which made the somatic embryo conservation easier. The addition of activated charcoal could increase the secondary somatic embryo induction rate but lowered the quantity of the induction. And the dedifferentiation step was achieved from different stage of the embryos. The optimal secondary somatic embryo induce treatment was obtained, which lays the foundation for genetic transformation.
