<P>大梅与梅山猪背最长肌正反消减文库的构建及序列分析</P>

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摘要为从基因差异表达的角度研究杂种优势的分子机理，构建了差减效率均为210倍的大梅与梅山猪背最长肌正反消减文库。在大梅(被消减)-梅山库中挑选了610个有效克隆，经斑点杂交筛选出48个阳性克隆，测序后经Blastn网上比对揭示了37个基因，其中28个EST是已报道基因的cDNA片段，9个在NCBI数据库中没有同源序列。在梅山(被消减)-大梅挑选了580个有效克隆，经斑点杂交筛选出45个阳性克隆，测序后经Blastn网上比对揭示了34个基因，其中26个EST是已报道基因的cDNA片段，8个在NCBI数据库中没有同源序列。

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	黄涛
	熊远著
	徐德全
	邓昌彦
	雷明刚
	蒋思文
	郑嵘
	姜勋平
	李凤娥
	李家连
	左波

关键词 ：抑制消减杂交, 杂种优势, 消减cDNA文库, 肌肉组织, 表达序列标签, 基因

Abstract：In order to discover the molecular mechanism of heterosis from the poit of gene differential expression, forward and reverse subtracted cDNA libraries were constructed between longissimus muscle tissue of Meishan and Large White×Meishan crossbred pigs both with an efficiency of 210 times. Six hundred and ten avilable clones were selected in the library between Large White×Meishan crossbred (subtracted) and Meishan pigs. Forty-eight positive clones were selected by Dot blotting method. The sequencing results revealed that 28 ESTs of 37 genes represented known genes respectively. The nine ESTs had no homology from NCBI. Five hundred and eighty avilable clones were selected in the library between Meishan (subtracted) and Large White×Meishan crossbred pigs. Forty-five positive clones were selected by Dot blotting method. The sequencing results revealed that 26 ESTs of 34 genes represented reported genes respectively. The eight ESTs had no homology from NCBI.

Key words： suppression subtractive hybridization(SSH) heterosis subtrated cDNA library muscle 　tissue expression sequence tag(EST) gene

收稿日期: 2005-06-22

ZTFLH:

S188

通讯作者: 熊远著

引用本文:

黄涛；熊远著；徐德全；邓昌彦；雷明刚；蒋思文；郑嵘；姜勋平；李凤娥；李家连；左波

大梅与梅山猪背最长肌正反消减文库的构建及序列分析

[J]. , 2006, 14(4): 456-461.

HUANGTao；XIONGYuan-zhu；XUDe-quan；DENGChang-yan；LEIMing-gang；JIANGSi-wen；ZHENGRong；JIANGXun-ping；LIFeng-e,LIJia-lian；ZUOBo

Construction of Forward and Reverse Subtracted cDNA Libraries between
Longissimus Muscle Tissue of Meishan and Large White×Meishan Crossbred
Pigs and ESTs Analysis

. , 2006, 14(4): 456-461.

链接本文:

http://journal05.magtech.org.cn/Jwk_ny/CN/ 或 http://journal05.magtech.org.cn/Jwk_ny/CN/Y2006/V14/I4/456