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2025年7月28日 星期一
  研究论文 本期目录 | 过刊浏览 | 高级检索 |
反义乙烯受体LeETR2基因对番茄的转化和功能分析
张明方1 向庆宁1 应铁进2** 杨虎清2 郑铁松3 杜荣茂2
(1. 浙江大学农业与生物技术学院,杭州 310029;2. 浙江大学农业工程与食品科学学院,杭州 310029;
3.南京师范大学女子学院,南京 210095)
Transformation and Functional Analysis of Antisense Ethylene
 Receptor LeETR2 Gene in  Tomato
Zhang Mingfang1 Xiang Qingning1 Ying Tiejin2 ** Yang Huqing2 Zheng Tiesong 3 Du Rongmao2
(1.College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310029,China;
2. College of Agricultural Engineering and Food Science, Zhejiang University, Hangzhou 310029,China;
3. College of Women, Nanjing Normal University, Nanjing 210095,China)
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摘要 摘要:采用根癌农杆菌(Agrobacterium tumefaciens)介导法将LeETR2的部分反义序列导入番茄(Lycopersicon esculentum)子叶外植体,经抗性筛选和组织培养获得再生植株。通过对转基因植株中插入序列的PCR检测和Southern杂交,证明转化番茄成功。通过对LeETR2的功能的初步研究结果表明, 转基因植株顶端优势丧失、极端矮化和番茄果实的内源乙烯合成量增加。这提示此基因在植物乙烯信号感受与转导系统中起着负调控的作用。
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张明方
向庆宁
应铁进
**杨虎清
郑铁松
杜荣茂
关键词 关键词:番茄乙烯受体 LeETR2基因 反义转化 功能    
AbstractAbstract: An antisense ethylene receptor gene LeETR2 was introduced into tomato (Lycopersicon esculentum ) through Agrobacberium tumefaciens -mediated method. Plantlets were regenerated in vitro by resistance selection on MS medium containing various concentrations of kanamycin. Approximately 73.5% of calli and 38.5% of leaves from plantlets exhibited intense blue color reaction by histochemical GUS assays. By PCR-aided assays, transformed plants displayed 362 bp (antisense LeEtr2 gene)and 500 bp(NPTⅡ) amplification products, however, they were not detected in non-transformed controls. Furthermore, Southern hybridization of DNA from PCR-positive transformants with the NPTⅡ probe indicated that the inserted gene was single copied in genome, by which it was further confirmed that the antisense LeETR2 gene was transferred into tomato. The transformants in tested lines showed delayed abscission, less elongated internodes(6±1.5) cm in length for transgenic plants and (8±1.58) cm for control, and out-growed axillary buds. The endogenous ethylene levels in fruits at day 3 after breaker in transgenic lines 1~5 were 155.58%, 166.96%, 251.76%, 441.07% and 100.89% of those in the controls, respectively. It is still ambiguous that how ethylene interacts with other hormones, such as auxin, abscisic acid and cytokinin in regulation of plant development through signal pathways. Nevertheless, the ethylene receptor LeETR2 might play a negative regulatory role in the signal transduction, and possibly involve other hormones in the control of plant development. It is essential to make clear how the antisense LeETR2  gene acts at transcriptional or translational level in the regulation of ethylene receptor LeETR2 gene, and whether it has regulatory side-effect on ethylene receptor LeETR1 gene due to their high sequence homologies.
Key wordsKey words:tomato    ethylene receptor    LeETR2 gene    antisense transformation    function
收稿日期: 1900-01-01     
引用本文:   
张明方1 向庆宁1 应铁进2** 杨虎清2 郑铁松3 杜荣茂2. 反义乙烯受体LeETR2基因对番茄的转化和功能分析[J]. , 2003, 11(2): 144-147.
Zhang Mingfang1 Xiang Qingning1 Ying Tiejin2 ** Yang Huqing2 Zheng Tiesong 3 Du Rongmao2. Transformation and Functional Analysis of Antisense Ethylene
 Receptor LeETR2 Gene in  Tomato
. , 2003, 11(2): 144-147.
链接本文:  
http://journal05.magtech.org.cn/Jwk_ny/CN/      或     http://journal05.magtech.org.cn/Jwk_ny/CN/Y2003/V11/I2/144
 
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