Abstract：Myo-inositol-1-phosphate synthase (MIPS) is a key rate-limiting enzyme in inositol biosynthetic, and plays important roles in plant growth regulation and abiotic stress response. To investigate fuction of MIPS in grape (Vitis vinifera), the full-length cDNA of VvMIPS was cloned from cultivar Zuoyouhong. Sequence analysis showed that the size of VvMIPS was 1 533 bp (GenBank No. 1984676), encoding a protein of 510 amino acids with molecular weight of 56.3 kD, and isoelectric point of 5.37. VvMIPS presented high similarity with MIPS proteins from Glycine max, Medicago sativa and Trifolium pratense. qRT-PCR analysis showed the expression of VvMIPS was the highest in flower buds, stems, and the lowest in root. In addition, VvMIPS was highly induced by low and high temperature stress, and induced by H2S, abscisic acid (ABA) and H2O2. The recombinant plasmid pET28a-VvMIPS was transformed into Escherichia coli BL21, and expression of VvMIPS protein was tested. The results showed that VvMIPS protein could enhance the resistance of E.coli to low and high temperature stress. This study provides the genetic resources for grape cultivars improvement with lower or higher temperature stress tolerance.
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