Identification of the VQ Gene Family in Cabbage (Brassica oleracea var. capitata) and Their Expression Analysis in Response to the Infection by Plasmodiophora brassicae
WANG Min, CHEN Jin-Xiu, TAI Xiang, ZHU Xiao-Wei, REN Yun-Ying, BO Tian-Yue*
Institute of Horticulture, Shanghai Academy of Agricultural Science/Shanghai Key Lab of Protected Horticultural Technology, Shanghai 201403, China
Abstract：VQ protein, a class of amino acid sequences containing “FxxxVQxLTG” conservative motifs, is a plant specific protein family, which participated in plant growth, development, and various stress response. In order to clarify the biological function of the VQ gene family in cabbage, 67 BoVQ genes were identified from the whole genome of cabbage, and their bioinformatics and expression patterns were analyzed. The phylogenetic analysis showed that BoVQ genes were divided into 7 subgroups. Chromosomes mapping exhibited BoVQ genes were unevenly distributed in single or cluster on 9 chromosomes of cabbage. The transcriptome data suggested that BoVQ genes expressions varied at different tissues. Eleven BoVQ genes (BoVQ10, BoVQ20, BoVQ22, BoVQ32, BoVQ36, BoVQ43, BoVQ49, BoVQ54, BoVQ59, BoVQ60 and BoVQ67) participated in the whole process of cabbage flower development. The results of real-time fluorescence quantitative PCR (qRT-PCR) showed that BoVQ2, BoVQ12, BoVQ21, BoVQ26 and BoVQ44 genes were significantly high expressed under the infection of clubroot pathogen. Prediction of cis-elements revealed that the pathogen response element W-box and the defense and stress response element TC-rich repeats were enriched in the promoter of BoVQ genes in response to Plasmodiophora brassicae. Protein interaction network prediction showed that there were complex interactions between BoVQ proteins. This study provides a reference for further exploring the function and evolution of BoVQ genes in cabbage.
王敏, 陈锦秀, 邰翔, 朱晓炜, 任云英, 薄天岳. 甘蓝VQ基因家族鉴定及其响应根肿病菌侵染的表达分析[J]. 农业生物技术学报, 2023, 31(9): 1786-1803.
WANG Min, CHEN Jin-Xiu, TAI Xiang, ZHU Xiao-Wei, REN Yun-Ying, BO Tian-Yue. Identification of the VQ Gene Family in Cabbage (Brassica oleracea var. capitata) and Their Expression Analysis in Response to the Infection by Plasmodiophora brassicae. 农业生物技术学报, 2023, 31(9): 1786-1803.
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