Identification of NAC Family Genes in Cajanus cajan and Analysis of Their Response to Fungal Infection
ZHANG Xiu-Qi1*, WU Rui1*, DONG Bi-Ying1, DU Ting-Ting1, SONG Zhi-Hua1, LI Na1, CAO Hong-Yan1, YANG Qing1,2, MENG Dong1,2**
1 The Key Laboratory for Silviculture and Conservation of Ministry of Education, College of Forestry, Beijing Forestry University, Beijing 100083, China; 2 Institute of Tree Development and Gene Editing, Beijing Forestry University, Beijing 100083, China
Abstract：The NAC (NAM, ATAF1/2 and CUC2) transcription factor family is a collective name for genes with conserved NAC structural domains that play key regulatory roles in plant growth, development, and stress response and other life activities. In this study, 90 CcNAC candidate genes were initially identified from the Cajanus cajan genome by sequence alignment using the NAC family genes of Arabidopsis thaliana. Phylogenetic analysis showed that the 90 CcNAC genes could be divided into 13 subfamilies, and a total of 24 CcNAC family genes were screened as potential biotic stress response genes in subfamilies Group1~4 based on the study of Arabidopsis gene functions. Further physicochemical characterization and cis-acting element analysis of the 24 CcNAC family genes showed that the promoter region contains several phytohormone response elements, which might be closely related to hormone signaling and have potential responses to biotic and abiotic stresses function. Transcriptome analysis showed that 13 NAC genes were significantly up- regulated and 5 NAC genes were significantly down-regulated at 12 h of treatment under the effect of MeJA, among which CcNAC55 was significantly up-regulated at 3, 6 and 12 h of treatment, and the highest expression levels were found at 6 and 12 h. Further, using semi-quantitative assays, CcNAC55 was found that it was sustaining significantly up-regulated under fungal Cc1-1 stress, while there was no significant difference under high temperature stress, suggested that CcNAC55 might play an important role in the response of Cajanus cajan to biotic stress. To verify the function of CcNAC55 in response to biotic stress, in this study, the lines of Cajanus cajan overexpressed CcNAC55 were established by vacuum osmotic transformation and inoculated the leaves with the pathogenic fungus Cc1-1. The results showed that CcNAC55 overexpression could significantly reduce the infestation rate and proportion of infected area of Cajanus cajan leaves. This study provides a reference for the study of NAC gene function in Cajanus cajan and will help to identify and select candidate genes related to biotic stress tolerance.
张修齐, 吴睿, 董碧莹, 杜婷婷, 宋治华, 李娜, 曹红燕, 杨清, 孟冬. 木豆 NAC 家族基因鉴定及其在真菌侵染下的响应分析[J]. 农业生物技术学报, 2023, 31(5): 927-942.
ZHANG Xiu-Qi, WU Rui, DONG Bi-Ying, DU Ting-Ting, SONG Zhi-Hua, LI Na, CAO Hong-Yan, YANG Qing, MENG Dong. Identification of NAC Family Genes in Cajanus cajan and Analysis of Their Response to Fungal Infection. 农业生物技术学报, 2023, 31(5): 927-942.
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