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2025年8月12日 星期二
农业生物技术学报  2023, Vol. 31 Issue (5): 943-957    DOI: 10.3969/j.issn.1674-7968.2023.05.005
  研究论文与报告 本期目录 | 过刊浏览 | 高级检索 |
桂花 AQP 基因家族鉴定及在花开放阶段的表达分析
周杰, 钟诗蔚, 方遒, 董彬, 王艺光, 肖政, 赵宏波*
浙江农林大学 风景园林与建筑学院/浙江省园林植物种质创新与利用重点实验室/南方园林植物种质创新与利用国家林业和草原局重点 实验室,杭州 311300
Identification of AQP Gene Family in Osmanthus fragrans and Its Expression Analysis During Flower Opening Stage
ZHOU Jie, ZHONG Shi-Wei, FANG Qiu, DONG Bin, WANG Yi-Guang, XIAO Zheng, ZHAO Hong-Bo*
School of Landscape and Architecture/Zhejiang Key Laboratory of Garden Plant Germplasm Innovation and Utilization/ Key Laboratory of Southern Garden Plant Germplasm Innovation and Utilization, State Forestry and Grassland Administration, Zhejiang A&F University, Hangzhou 311300, China
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摘要 水通道蛋白(aquaporin, AQP)是一种小分子膜内在蛋白,介导细胞与介质之间快速且被动的水分运输。本研究以桂花(Osmanthus fragrans) 为材料,基于桂花基因组数据库,依据非冗余蛋白库 (non- redundant protein sequence database, Nr)注释筛选获得桂花 AQP 家族 41 个成员的基因序列,通过系统进化分析将其划分为4个亚族:16个质膜内在蛋白(plasma membrane intrinsic proteins, PIP)、14 个液泡膜内在蛋白(tonoplast intrinsic proteins, TIP)、8 个类 NOD26 膜内在蛋白(nodulin-26-like intrinsic proteins, NIP)和3个小分子碱性膜内在蛋白(small intrinsic proteins, SIP)。同一亚族成员在基因结构和保守基序等方面具有较高保守性, 其编码蛋白长度为 145~432 个氨基酸; 分子量为 14.81~45.90 kD;等电点为 4.84~9.81。 qPCR 分析 OfPIPs 和 OfTIPs 在桂花不同组织中的表达模式发现,OfPIP1OfPIP5OfPIP12OfPIP13OfPIP14OfPIP15OfPIP16OfTIP1OfTIP3OfTIP4OfTIP6OfTIP9OfTIP13 在花中呈现特异性表达, 进一步对其在桂花花开放进程中的表达模式研究发现,OfTIP6 在花开放前期(S1~S2)有较高转录,推测其对花开放初期液泡的扩展发挥重要功能。而 OfPIP1OfPIP13OfPIP14OfPIP15OfPIP16OfTIP13 在桂花花开放的后期(S4~S5)相对表达量显著上调(P<0.05),推测这6个基因是在花开放后期行使功能的关键基因。本研究为进一步解析 OfAQPs 蛋白的生物学功能提供参考。
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周杰
钟诗蔚
方遒
董彬
王艺光
肖政
赵宏波
关键词 桂花水通道蛋白(AQP)花开放    
Abstract:Aquaporin (AQP) is a small molecule membrane intrinsic protein, which mediates the rapid and passive water transport and participates in transmembrane transport of plants. In this study, 41 genes of AQP gene family were identified according to non-redundant protein sequence database (Nr) annotation from the whole genome of Osmanthus fragrans. According to the analysis of amino acid sequence and phylogenetic tree, they were classified into 4 subgroups: 16 members of plasma membrane intrinsic proteins (PIP), 14 members of tonoplast intrinsic proteins (TIP), 8 members of nodulin-26-like intrinsic proteins (NIP), 3 members of small intrinsic proteins (SIP). The gene structure and conserved motif of amino acids in the same subgroup were highly conserved. The length of the AQP proteins ranged from 145 to 432 aa with molecular mass of 14.81 to 45.90 kD and the isoelectric points were from 4.84 to 9.81. The analysis of the expression patterns of OfPIPs and OfTIPs in different tissues of O. fragrans using qPCR showed that OfPIP1, OfPIP5, OfPIP12, OfPIP13, OfPIP14, OfPIP15, OfPIP16, OfTIP1, OfTIP3, OfTIP4, OfTIP6, OfTIP9 and OfTIP13 were specific expressed in flowers. Further study on their expression patterns during flower opening period of O. fragrans showed that, OfTIP6 was highly transcribed in the early flower opening period (S1~S2) of O. fragrans. It is speculated that OfTIP6 plays an important role in vacuole expansion at the early flower opening stage. However, the relative expression levels of OfPIP1, OfPIP13, OfPIP14, OfPIP15, OfPIP16 and OfTIP13 were significantly up-regulated at the late flower opening period (S4~S5) of O. fragrans (P<0.05). It is speculated that they are the key genes to exercise function at the late flower opening stage. This research provides a reference for further analyzing the biological function of OfAQPs protein.
Key wordsOsmanthus fragrans    Aquaporin (AQP)    Flower opening
收稿日期: 2022-03-07     
ZTFLH:  S685.13  
基金资助:国家自然科学基金(32072615)
通讯作者: *zhaohb@zafu.edu.cn   
引用本文:   
周杰, 钟诗蔚, 方遒, 董彬, 王艺光, 肖政, 赵宏波. 桂花 AQP 基因家族鉴定及在花开放阶段的表达分析[J]. 农业生物技术学报, 2023, 31(5): 943-957.
ZHOU Jie, ZHONG Shi-Wei, FANG Qiu, DONG Bin, WANG Yi-Guang, XIAO Zheng, ZHAO Hong-Bo. Identification of AQP Gene Family in Osmanthus fragrans and Its Expression Analysis During Flower Opening Stage. 农业生物技术学报, 2023, 31(5): 943-957.
链接本文:  
http://journal05.magtech.org.cn/Jwk_ny/CN/10.3969/j.issn.1674-7968.2023.05.005     或     http://journal05.magtech.org.cn/Jwk_ny/CN/Y2023/V31/I5/943
 
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